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Microbial metabolism of 2-chlorophenol, phenol and rho-cresol by Rhodococcus erythropolis M1 in co-culture with Pseudomonas fluorescens P1.
Microbiological Research ( IF 6.7 ) Pub Date : 2005-05-11 , DOI: 10.1016/j.micres.2004.10.004
M Goswami 1 , N Shivaraman , R P Singh
Affiliation  

Chlorophenolic waste most often contains phenol and rho-cresol along with chlorophenols. A Rhodococcus erythropolis strain M1 was isolated with the ability to degrade 2-chlorophenol, phenol and p-cresol (100 mgl(-1), each) in 18, 24 and 20 h, respectively, with negligible lag. However, Rhodococcus sp. characterized by low growth rate, pose a threat to be outgrown by bacteria occurring in natural habitats. In the present study, interaction of R. erythropolis M1 with another isolated bacteria generally encountered in activated sludge for water treatment like Pseudomonas fluorescens P1 was studied. 2-chlorophenol, phenol and p-cresol were selected as the substrates for the study. Viable cell counts showed competitive interaction between the species on 2-chlorophenol and phenol. Specific growth rate of pure culture of R. erythropolis M1 was higher than P. fluorescens P1 on 2-chlorophenol. However, in mixed culture, P. fluorescens P1 showed higher growth rate. Degradation of phenol showed higher growth rate of R. erythropolis M1 both in pure and in mixed culture form. Degradation of p-cresol had shown similar counts for both populations indicating neutral type of interaction. This observation was substantiated by detecting the growth rate, where both cultures had similar growth rate in pure and in the mixed culture form. Rate of 2-chlorophenol degradation was higher when R. erythropolis M1 was used as the pure culture as compared to the degradation rates observed with the P. fluorescens P1 or with the mixed culture. However, in case of phenol and p-cresol, degradation by the mixed culture had resulted in higher degradation rates as compared to the degradation of the substrates by both the axenic cultures.

中文翻译:

红球红球菌M1与荧光假单胞菌P1共培养时2-氯苯酚,苯酚和对甲酚的微生物代谢。

氯酚废物最常含有苯酚和对甲酚以及氯酚。分离出红球菌红霉素菌株M1,其具有分别在18、24和20小时内降解2-氯苯酚,苯酚和对甲酚(分别为100 mgl(-1))的能力,且滞后可以忽略不计。然而,红球菌属。其特点是生长速度慢,威胁到自然栖息地中细菌的生长。在本研究中,研究了R. erythropolis M1与活性污泥中通常遇到的另一种分离的细菌之间的相互作用,这些活性污泥用于水处理,例如荧光假单胞菌P1。选择2-氯苯酚,苯酚和对甲酚作为研究的底物。活细胞计数显示该物种在2-氯苯酚和苯酚之间存在竞争性相互作用。R的纯培养物的比生长率 在2-氯苯酚上,erythropolis M1高于荧光假单胞菌P1。然而,在混合培养中,荧光假单胞菌P1显示出更高的生长速率。无论是纯培养形式还是混合培养形式,苯酚的降解均显示出较高的R. erythropolis M1生长速率。对甲酚的降解显示出两个种群的计数相似,表明相互作用的类型是中性的。通过检测生长速率证实了该观察结果,其中两种培养物在纯培养物和混合培养物中的生长率均相似。当使用R. erythropolis M1作为纯培养物时,2-氯苯酚的降解率高于荧光假单胞菌P1或混合培养物中观察到的降解率。但是,如果是苯酚和对甲酚,
更新日期:2019-11-01
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