The study examined the diversity of ectomycorrhizal fungi, naturally established on roots of containerised Pinus seedlings in a nursery, using PCR-RFLP and sequencing of the nuclear ribosomal internal transcribed spacer. Seventy-two samples, including ectomycorrhizae and fruit bodies, were examined. Molecular typing assigned the fungal symbionts to four ectomycorrhizal Boletales: Rhizopogon rubescens, Suillus bovinus, S. variegatus, and R. luteolus. R. rubescens was abundant (37.5%), while Suillus and R. luteolus species were moderately established (25-26%) and rare (2.8%), respectively. In addition, Rhizopogon species colonised P. nigra ssp. salzmannii seedlings, whereas Suillus species were identified on Pinus nigra ssp. nigra seedlings. The diversity and the ability of these naturally established symbionts under artificial nursery conditions were discussed. The molecular survey investigated here should contribute to successful monitoring of mycorrhizal application under both nursery and plantation conditions.

中文翻译：

---

在苗圃条件下，在容器化松树幼苗上自然建立的外生菌根真菌的多样性。

该研究使用PCR-RFLP和核糖体内部转录间隔子的测序方法，对苗圃中带容器的松树幼苗的根部自然建立的外生菌根真菌的多样性进行了研究。检查了72个样品，包括外生菌根和子实体。分子分型法将真菌共生菌分配给了四个根除菌根的牛肝菌：根瘤菌，牛肝菌，变种沙门氏菌和黄褐菌。冬凌草（R. rubescens）丰富（37.5％），而牛肝菌（Suillus）和黄腐鼠（R. luteolus）种类分别中等（25-26％）和稀有（2.8％）。此外，根瘤菌菌种也定居在黑假单胞菌中。萨尔茨曼（Salzmannii）幼苗，而在黑松（Pinus nigra ssp）上发现了Suillus种。老黑的幼苗。这些天然建立的共生体在人工育苗条件下的多样性和能力进行了讨论。此处调查的分子调查应有助于成功监测苗圃和人工林条件下的菌根施用情况。