Thirty nosocomial isolates of Klebsiella pneumoniae, collected from three public hospitals in Recife, Brazil, between 1999 and 2000, were analysed in order to determine their epidemiological relatedness and genetic characteristics. The isolates were characterised by biotyping, antibiotyping, protein analysis, plasmid profile and random amplified polymorphic DNA (RAPD). The majority of the clinical isolates were resistant to multiple antibiotics, in particular to beta-lactams, and 30% were found to be ESBLs producers. RAPD proved to be the most effective technique in discriminating unrelated K. pneumoniae isolates. It was confirmed by the highly genetic similarity found among related isolates from an hospital outbreak. We conclude that K. pneumoniae infections in Recife has been caused by a variety of bacterial genotypes. This is the first report that revealed the subtypes of K. pneumoniae in Brazil by plasmid analysis and RAPD.

中文翻译：

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巴西累西腓公立医院肺炎克雷伯菌分离株的分子分型。

为了确定它们的流行病学相关性和遗传特征，分析了1999年至2000年从巴西累西腓的三家公立医院收集的30株肺炎克雷伯菌的医院分离株。通过生物分型，抗生物分型，蛋白质分析，质粒图谱和随机扩增多态性DNA（RAPD）表征分离株。大多数临床分离株对多种抗生素具有抵抗力，特别是对β-内酰胺类有抵抗力，并且发现30％是ESBLs生产者。RAPD被证明是区分无关的肺炎克雷伯菌分离物的最有效技术。医院暴发的相关分离株之间发现的高度遗传相似性证实了这一点。我们得出的结论是累西腓的肺炎克雷伯菌感染是由多种细菌基因型引起的。