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Erythropoietic changes in rats after 2.45 GJz nonthermal irradiation.
International Journal of Hygiene and Environmental Health ( IF 6 ) Pub Date : 2005-02-26 , DOI: 10.1078/1438-4639-00326
Ivana Busljeta 1 , Ivancica Trosic , Sanja Milkovic-Kraus
Affiliation  

The purpose of this study was to observe the erythropoietic changes in rats subchronically exposed to radiofrequency microwave (RF/MW) irradiation at nonthermal level. Adult male Wistar rats (N=40) were exposed to 2.45 GHz continuous RF/MW fields for 2 hours daily, 7 days a week, at 5-10 mW/cm2. Exposed animals were divided into four subgroups (n=10 animals in each subgroup) in order to be irradiated for 2, 8, 15 and 30 days. Animals were sacrified on the final irradiation day of each treated subgroup. Unexposed rats were used as control (N=24). Six animals were included into the each control subgroup. Bone marrow smears were examined to determine absolute counts of anuclear cells and erythropoietic precursor cells. The absolute erythrocyte count, haemoglobin and haematocrit values were observed in the peripheral blood by an automatic cell counter. The bone marrow cytogenetic analysis was accomplished by micronucleus (MN) tests. In the exposed animals erythrocyte count, haemoglobin and haematocrit were increased in peripheral blood on irradiation days 8 and 15. Concurrently, anuclear cells and erythropoietic precursor cells were significantly decreased (p < 0.05) in the bone marrow on day 15, but micronucleated cells' frequency was increased. In the applied experimental condition, RF/MW radiation might cause disturbance in red cell maturation and proliferation, and induce micronucleus formation in erythropoietic cells.

中文翻译:

2.45 GJz非热辐射后大鼠的红细胞生成变化。

这项研究的目的是观察在非热水平下亚慢性暴露于射频微波(RF / MW)照射的大鼠的红细胞生成变化。成年雄性Wistar大鼠(N = 40)每周7天每天以5-10 mW / cm2的频率暴露于2.45 GHz连续RF / MW场中2小时。将暴露的动物分成四个亚组(每个亚组中n = 10只动物),以便对其照射2、8、15和30天。在每个处理的亚组的最后辐射日处死动物。未暴露的大鼠用作对照(N = 24)。每个对照亚组中包括六只动物。检查骨髓涂片以确定绝对数量的核细胞和促红细胞生成前体细胞。通过自动细胞计数器在外周血中观察到绝对红细胞计数,血红蛋白和血细胞比容值。骨髓细胞遗传学分析通过微核(MN)测试完成。在暴露的动物中,第8天和第15天的照射后,外周血中红细胞计数,血红蛋白和血细胞比容增加。同时,第15天,骨髓中的核细胞和促红细胞生成前体细胞显着减少(p <0.05),但微核细胞频率增加了。在应用的实验条件下,RF / MW辐射可能会干扰红细胞的成熟和增殖,并诱导红细胞生成中的微核形成。第15天,骨髓中的核细胞和促红细胞生成前体细胞显着减少(p <0.05),但微核细胞的频率增加。在应用的实验条件下,RF / MW辐射可能会干扰红细胞的成熟和增殖,并诱导红细胞生成中的微核形成。第15天,骨髓中的核细胞和促红细胞生成前体细胞显着减少(p <0.05),但微核细胞的频率增加。在应用的实验条件下,RF / MW辐射可能会干扰红细胞的成熟和增殖,并诱导红细胞生成中的微核形成。
更新日期:2019-11-01
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