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Trypanosoma cruzi infection disrupts vinculin costameres in cardiomyocytes.
European Journal of Cell Biology ( IF 6.6 ) Pub Date : 2005-02-01 , DOI: 10.1078/0171-9335-00419
Tatiana G Melo 1 , Danielle S Almeida , Maria de Nazareth S L de Meirelles , Mirian Claudia Pereira
Affiliation  

Chagas' disease cardiomyopathy is an important manifestation of Trypanosoma cruzi infection, leading to cardiac dysfunction and serious arrhythmias. We have here investigated by indirect immunofluorescence assay the distribution of vinculin, a focal adhesion protein with a major role in the transmission of contraction force, during the T. cruzi-cardiomyocyte infection in vitro and in vivo. No change in vinculin distribution was observed after 24 h of infection, where control and T. cruzi-infected cardiomyocytes displayed vinculin localized at costameres and intercalated discs. On the other hand, a clear disruption of vinculin costameric distribution was noted after 72 h of infection. A significant reduction in the levels of vinculin expression was observed at all times of infection. In murine experimental Chagas' disease, alteration in the vinculin distribution was also detected in the infected myocardium, with no costameric staining in infected myocytes and irregular alignment of intercalated discs in cardiac fibers. These data suggest that the disruption of costameric vinculin distribution and the enlargement of interstitial space due to inflammatory infiltration may contribute to the reduction of transmission of cardiac contraction force, leading to alterations in the heart function in Chagas' disease.

中文翻译:

克鲁氏锥虫感染会破坏心肌细胞中的纽蛋白肋骨。

恰加斯氏病心肌病是克氏锥虫感染的重要表现,导致心脏功能障碍和严重的心律失常。在这里,我们通过间接免疫荧光试验研究了纽扣氏菌(一种在粘附力的传递中起主要作用的粘着斑蛋白)在体外和体内感染克鲁维氏梭菌的过程中的分布。感染后24小时,未观察到新蛋白的分布,对照组和克鲁斯氏菌感染的心肌细胞显示新蛋白定位在肋骨和插入椎间盘上。另一方面,在感染72小时后,发现新蛋白的弹性蛋白分布明显中断。在感染的所有时间都观察到新蛋白表达水平显着降低。在鼠实验性恰加斯病中,在感染的心肌中也检测到了纽蛋白分布的变化,在感染的心肌细胞中没有肋骨染色,并且在心脏纤维中没有插入盘的不规则排列。这些数据表明,由于炎性浸润导致的肋骨纽蛋白分布的破坏和间隙的扩大可能有助于减少心脏收缩力的传递,从而导致恰加斯病的心脏功能改变。
更新日期:2019-11-01
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