Agro-industrial wastes are excellent sources for solid-state culture to produce spores of microorganisms, whereas microbial co-cultivation is not fully exploited in solid-state culture. In this work, the co-cultivation of different strains of Bacillus subtilis, and three microbes of B. subtilis, Bacillus mucilaginosus, and Paecilomyces lilacinus was studied using a solid medium only composed of water and tobacco waste residue after extraction of nicotine and solanesol. The influences of matrix thickness, moister, temperature, and ratio of three microbes in seed on the cell growth and spore formation were studied. The maximum viable cells and spores of each microbe reached 1013 cfu/g when cultured alone at 30 °C in a medium containing 58.3% moisture. Co-cultivation of microbes stimulated cell growth and maximum viable cells of each microbe reached 1014 cfu/g, while spore production was inhibited and decreased to 1011 cfu/g. With decreasing amount of P. lilacinus in seed, total amount of spores was increased. When the seed with a ratio of 6:3:1 for B. mucilaginosus, B. subtilis, and P. lilacinus was inoculated, the total amount of spores reached 4.14 × 1012 cfu/g and the ratio was 1.7:0.7:1. These results indicate the potential of solid-state cultivation in the high production of spores from tobacco waste residue at low cost.

中文翻译：

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使用烟草废渣对枯草芽孢杆菌，粘液芽孢杆菌和淡紫拟青霉进行固态共培养。

农业工业废物是固态培养产生微生物孢子的极好来源，而固态培养并未充分利用微生物的共培养。在这项工作中，使用仅由水和烟草废渣组成的固体培养基，提取尼古丁和茄尼醇后，研究了不同菌株的枯草芽孢杆菌，枯草芽孢杆菌，粘液芽孢杆菌和淡紫色拟杆菌的三种微生物的共培养。研究了基质厚度，水分，温度以及种子中三种微生物的比例对细胞生长和孢子形成的影响。当在含水量为58.3％的培养基中于30°C单独培养时，每种微生物的最大活细胞和孢子达到1013 cfu / g。微生物的共培养刺激细胞生长，每个微生物的最大存活细胞达到1014 cfu / g，而孢子产生被抑制并降低到1011 cfu / g。随着种子中丁香假单胞菌数量的减少，孢子的总量增加。接种粘菌芽孢杆菌，枯草芽孢杆菌和淡紫青霉菌的比例为6：3：1的种子时，孢子总量达到4.14×1012 cfu / g，比例为1.7：0.7：1。这些结果表明固态种植具有潜力，可以以低成本从烟草废渣中大量生产芽孢。孢子总量达到4.14×1012 cfu / g，比例为1.7：0.7：1。这些结果表明固态种植具有潜力，可以以低成本从烟草废渣中大量生产芽孢。孢子总量达到4.14×1012 cfu / g，比例为1.7：0.7：1。这些结果表明固态种植具有潜力，可以以低成本从烟草废渣中大量生产芽孢。