A novel virus was discovered in a Rosa wichuraiana Crep. by high-throughput sequencing and tentatively named "rose virus A" (RVA). Based on sequence identity and phylogenetic analysis, RVA represents a new member of the genus Carlavirus (family Betaflexiviridae). The genome of RVA is 8,849 nucleotides long excluding the poly(A) tail and contains six open reading frames (ORFs). The predicted ORFs code for a replicase, triple gene block (TGB), coat protein, and nucleic acid binding protein, as in a typical carlavirus. RVA is the first carlavirus identified in rose and has the highest nucleotide sequence similarity to poplar mosaic virus. Reverse transcription-PCR-based assays were developed to confirm the presence of RVA in the original source and to screen additional rose plants.

中文翻译：

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玫瑰病毒A的完整基因组序列，这是玫瑰中鉴定出的第一个甲状病毒。

在Rosa wichuraiana Crep中发现了一种新型病毒。通过高通量测序并暂时命名为“玫瑰病毒A”（RVA）。基于序列同一性和系统发育分析，RVA代表了卡拉病毒属（Betaflexiviridae家族）的新成员。RVA的基因组长度为8,849个核苷酸（不包括poly（A）尾巴），并且包含六个开放阅读框（ORF）。预测的ORF编码复制酶，三重基因区块（TGB），外壳蛋白和核酸结合蛋白，就像典型的水痘病毒一样。RVA是玫瑰中鉴定出的第一种卡拉维病毒，与杨树花叶病毒的核苷酸序列相似性最高。开发了基于逆转录-PCR的测定法，以确认原始来源中是否存在RVA并筛选其他玫瑰植物。