Alzheimer's disease (AD) is the most common neurodegenerative disease, leading to dementia in the aging human brain. Although a huge amount of research has been done to date, the pathogenesis of AD remains largely unknown. Given the dominance of mRNA splicing in the nervous system, it would be invaluable to precisely identify mis-splicing in the AD brain to understand more deeply how the gene network orchestrates the development of pathology in affected cells. In this brief manuscript, we first introduce the term mRNA splicing and discuss its abnormality in the AD brain. Then, we describe the technical challenges of the precise detection of mis-splicing, as well as their reasonable solutions. Finally, we discuss the future directions for splicing biology in AD.

阿尔茨海默氏病（AD）是最常见的神经退行性疾病，可导致人脑衰老引起痴呆。尽管迄今为止已经进行了大量研究，但是AD的发病机理仍然未知。鉴于mRNA剪接在神经系统中的优势地位，准确鉴定AD脑中的错剪以更深入地了解基因网络如何在受影响的细胞中协调病理学发展将是无价的。在这份简短的手稿中，我们首先介绍术语mRNA剪接并讨论其在AD脑中的异常情况。然后，我们描述了精确检测错接的技术挑战及其合理的解决方案。最后，我们讨论了AD拼接生物学的未来方向。