Background: Polyphenol catechins from green tea, particularly (−)-epigallocatechin-3-gallate (EGCG), exhibits numerous beneficial health effects, although the mechanisms remain unclear.
Methods: In this study, the mechanism of EGCG-mediated healing in an experimentally injured zebrafish model was examined at the cellular and molecular level using confocal microscopy and gene expression analysis.
Results: The mechanisms of action of EGCG were shown to involve: (1) reducing neutrophil response (accumulation, travel speed, and distance) and (2) downregulating the expression of IL-1β, TNFα, and related signaling pathways. As determined by dynamic time-lapse tracking studies, the local accumulation of neutrophils with high migration speeds after wounding (n=33 cells, v=0.020 μm/s, d=37.8 μm), underwent significant reduction following treatment with EGCG doses of 300 μM (n=22 cells, v=0.013 μm/s, d=39.5 μm) and 600 μM (n=18 cells, v=0.008 μm/s, d=9.53 μm). Reverse transcription polymerase chain reaction studies revealed that several signature genes in the IL-1β, TNFα, and related signaling pathways were downregulated after EGCG treatment.
Conclusion: The convenience, transparency, and simplicity of the zebrafish model facilitate tracking of fluorescent neutrophils in real time, in order to monitor inflammation, and assess the impact of therapeutic agents.

Keywords: green tea, innate immunity, animal models, IL-1, TNF


中文翻译：

---

Epigallocatechin-3-gallate 抑制转基因斑马鱼模型中的中性粒细胞迁移速度，同时减少炎症介质。

背景：绿茶中的多酚儿茶素，特别是 (-)-epigallocatechin-3-gallate (EGCG)，表现出许多有益的健康影响，尽管机制仍不清楚。
方法：在这项研究中，使用共聚焦显微镜和基因表达分析在细胞和分子水平上检查了实验性受伤斑马鱼模型中 EGCG 介导的愈合机制。
结果：EGCG 的作用机制包括：（1）降低中性粒细胞反应（积累、行进速度和距离）和（2）下调 IL-1β、TNFα 和相关信号通路的表达。正如动态延时跟踪研究所确定的，在用 EGCG 剂量 300 治疗后，具有高迁移速度的中性粒细胞的局部积累（n = 33 个细胞，v = 0.020 μm/s，d = 37.8 μm）显着减少μM（n=22 个细胞，v=0.013 μm/s，d=39.5 μm）和 600 μM（n=18 个细胞，v=0.008 μm/s，d=9.53 μm）。逆转录聚合酶链反应研究表明，在 EGCG 处理后，IL-1β、TNFα 和相关信号通路中的几个特征基因被下调。
结论：斑马鱼模型的便利性、透明度和简单性有助于实时跟踪荧光中性粒细胞，以监测炎症并评估治疗剂的影响。

关键词：绿茶，先天免疫，动物模型，IL-1，TNF