Diarrhea is largely caused by dysbiosis accompanying the hyperproliferation of Escherichia coli (E. coli). While current treatments can resolve the symptoms, they cannot suppress the proliferation of pathogenic bacteria in the intestine. Probiotics have numerous beneficial effects on host health, including restoring the balance of the intestinal microbiota. This study investigated the effect of the probiotic Bifidobacterium bifidum G9-1 (BBG9-1), which is active in intestinal dysbiosis, in the incidence of diarrhea, in the composition of the intestinal microbiota, and in the intestinal tissue of a rat model of phytohemagglutinin (PHA)-induced diarrhea. The rats were treated with PHA, with and without BBG9-1, and the microbiota composition throughout the intestine and stool was examined using high-throughput 16S rRNA sequencing. In line with previous reports, PHA administration caused diarrhea as well as dysbiosis due to E. coli hyperproliferation. Histological findings indicated that the jejunal villus length was shortened. Rats that received BBG9-1 showed clear improvements in dysbiosis, diarrhea symptoms, and jejunal villus length. Principal coordinates analysis demonstrated the microbiota profile to be more similar between the BBG9-1 and normal groups than between the PHA and normal groups. These results indicated that BBG9-1 suppresses the hyperproliferation of E. coli and restores the jejunal villus length, thereby improving dysbiosis, and in turn, alleviating the symptoms of diarrhea.

中文翻译：

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益生菌双歧双歧杆菌G9-1改善了由植物血凝素引起的肠道营养不良引起的腹泻。

腹泻主要是由伴随大肠杆菌（E. coli）过度增殖的营养不良引起的。尽管目前的治疗方法可以缓解症状，但不能抑制肠道中病原菌的增殖。益生菌对宿主健康具有许多有益的作用，包括恢复肠道菌群的平衡。这项研究调查了益生菌双歧双歧杆菌G9-1（BBG9-1）在肠道营养不良，腹泻的发生率，肠道菌群的组成以及鼠模型的肠道组织中的作用。植物血凝素（PHA）引起的腹泻。用PHA（有或没有BBG9-1）对大鼠进行治疗，并使用高通量16S rRNA测序检查了整个肠道和粪便中的微生物群组成。与以前的报道一致，由于大肠杆菌的过度增殖，PHA给药引起腹泻和营养不良。组织学结果表明空肠绒毛长度缩短。接受BBG9-1的大鼠在营养不良，腹泻症状和空肠绒毛长度方面表现出明显的改善。主坐标分析表明，BBG9-1和正常组之间的微生物群谱比PHA和正常组之间的菌群谱更相似。这些结果表明，BBG9-1抑制了大肠杆菌的过度增殖并恢复了空肠绒毛的长度，从而改善了营养不良，进而减轻了腹泻的症状。接受BBG9-1的大鼠在营养不良，腹泻症状和空肠绒毛长度方面表现出明显的改善。主坐标分析表明，BBG9-1和正常组之间的微生物群谱比PHA和正常组之间的菌群谱更相似。这些结果表明，BBG9-1抑制了大肠杆菌的过度增殖并恢复了空肠绒毛的长度，从而改善了营养不良，进而减轻了腹泻的症状。接受BBG9-1的大鼠在营养不良，腹泻症状和空肠绒毛长度方面表现出明显的改善。主坐标分析表明，BBG9-1和正常组之间的微生物群谱比PHA和正常组之间的菌群谱更相似。这些结果表明，BBG9-1抑制了大肠杆菌的过度增殖并恢复了空肠绒毛的长度，从而改善了营养不良，进而减轻了腹泻的症状。