The application of isotope tracers for investigating metabolism in mice is discussed. To familiarize the reader, some basic principles regarding the use of tracer methods are outlined. Emphasis is placed on showing how investigators are using isotope tracers to study the regulation of carbohydrate, fat and/or protein turnover in vivo. Finally, some of the advantages of using labeled water (i.e., 2H(2)O and/or H(2)18O) to trace the kinetics of biological processes are considered. The background provided in this report should assist engineers in designing studies that enhance our understanding of conditions in which metabolism is altered (e.g., diabetes, cancer cachexia, failure to thrive and travel at zero-gravity).

中文翻译：

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使用同位素示踪剂研究代谢：在小鼠模型中的应用。

讨论了同位素示踪剂在小鼠代谢研究中的应用。为了使读者熟悉，概述了有关使用示踪剂方法的一些基本原理。重点放在显示研究人员如何使用同位素示踪剂来研究体内碳水化合物，脂肪和/或蛋白质更新的调节。最后，考虑了使用标记水（即2H（2）O和/或H（2）18O）跟踪生物过程动力学的一些优点。本报告提供的背景信息应有助于工程师设计研究，以加深我们对新陈代谢发生变化的条件的理解（例如，糖尿病，癌症恶病质，壮成长和零重力运动）。