当前位置: X-MOL 学术Curr. Opin. Chem. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Recent improvements in antigene technology.
Current Opinion in Chemical Biology ( IF 7.8 ) Pub Date : 2003-12-04 , DOI: 10.1016/j.cbpa.2003.10.007
Sabrina Buchini 1 , Christian J Leumann
Affiliation  

DNA triple-helix-based approaches to control and modulate cellular functions on the level of genomic DNA (antigene technology) suffered in the past from a stepmother-like treatment in comparison to the flourishing field of oligonucleotide-based control of translation (antisense technology). This was mostly due to lack of affinity of triplex-forming oligonucleotides to their DNA target, to sequence restriction constraints imposed by the triple helical recognition motifs and by open questions to the accessibility of the target DNA. Recent developments in the area have brought about new bases that specifically recognize pyrimidine-purine inversion sites as well as sugar modifications, for example, the 2'-aminoethoxy-oligonucleotides or oligonucleotides based on the locked nucleic acid sugar unit, which greatly enhance triplex stability and alleviate in part the sequence restriction constraints. With this, sequence-specific genomic DNA manipulation is starting to become a useful tool in biotechnology.

中文翻译:

抗原技术的最新改进。

与基于寡核苷酸的翻译控制蓬勃发展的领域(反义技术)相比,基于DNA三螺旋的方法在基因组DNA水平上控制和调节细胞功能(抗原技术)在过去曾遭受过继母般的处理。 。这主要是由于形成三链体的寡核苷酸对其DNA靶标缺乏亲和力,由于三链螺旋识别基序和对靶标DNA可及性的开放性问题而施加的序列限制约束。该领域的最新发展带来了新的碱基,这些碱基可以特异性识别嘧啶-嘌呤的转化位点以及糖修饰,例如2'-氨基乙氧基-寡核苷酸或基于锁定核酸糖单元的寡核苷酸,这大大提高了三链体的稳定性,并部分缓解了序列限制的约束。由此,序列特异性基因组DNA操作开始成为生物技术中的有用工具。
更新日期:2019-11-01
down
wechat
bug