A bacterial isolate, with an optimum growth temperature of about 50 degrees C, was recovered from a domestic hot water tank in Coimbra. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain CLN-1T is a member of the beta-Proteobacteria and represents a new species of the genus Tepidimonas. The major fatty acids of strain CLN-1T are 16:0, 17:0 cyclo and 16:1 omega7c. Ubiquinone 8 is the major respiratory quinone, the major polar lipids are phosphatidylethanolamine, and phosphatidylglycerol. The new isolate is aerobic and facultatively chemolithoheterotrophic. Thiosulfate and tetrathionate are oxidized to sulfate in the presence of a metabolizable carbon source. Strain CLN-1T grows on amino acids and organic acids, but this organism does not assimilate carbohydrates. Glycerol is the only polyol assimilated. Resinic acids, namely abietic acid, dehydroabietic acid and isopimaric acid are not degraded. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we propose that strain CLN-1T represents a new species for which we offer the name Tepidimonas aquatica.

中文翻译：

---

水生Tepidimonas sp。nov。，一种从热水罐中分离出来的新的略带嗜热性的β-变形杆菌。

从科英布拉的一个家用热水箱中回收了最佳生长温度约为50摄氏度的细菌分离株。使用16S rRNA基因序列的系统发育分析表明，菌株CLN-1T是β-变形杆菌的成员，代表了新的Tepidimonas属。菌株CLN-1T的主要脂肪酸为16：0、17：0环和16：1 omega7c。泛醌8是主要的呼吸道醌，主要的极性脂质是磷脂酰乙醇胺和磷脂酰甘油。新的分离物是有氧的，兼具化学异养性。在可代谢的碳源存在下，硫代硫酸盐和四硫代酸盐被氧化为硫酸盐。CLN-1T菌株生长在氨基酸和有机酸上，但这种生物不会吸收碳水化合物。甘油是唯一被同化的多元醇。树脂酸 即松香酸，脱氢松香酸和异海藻酸不被降解。根据系统发育分析，生理和生化特征，我们提出菌株CLN-1T代表了一个新物种，为此我们将其命名为Tepidimonas aquatica。