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A Leishmania donovani dominant-negative mutant for eIF2α kinase LdeK1 elicits host-protective immune response.
Parasite Immunology ( IF 2.2 ) Pub Date : 2019-11-06 , DOI: 10.1111/pim.12678
Mukesh Kumar Jha 1 , Shilpa J Rao 2 , Aditya Y Sarode 1 , Bhaskar Saha 1, 3 , Anita Kar 2 , Jayanta K Pal 4
Affiliation  

Dominant-negative mutation of LdeK1 gene, an eIF2α kinase from Leishmania donovani, revealed its role in translation regulation in response to nutrient starvation earlier. However, whether the kinase influences the infectivity of the parasites which naturally encounters nutrient deprivation during its life cycle was interesting to investigate. Both in vitro and in vivo experiments resulted in decrease of the parasite burden in peritoneal macrophages and in splenic/ hepatic load, respectively. An insight into the immune response of mice infected with mutant parasite showed enhanced pro-inflammatory cytokines and nitric oxide levels but reduced TH 2 and Treg population. The significantly reduced loss of infectivity of the parasites lacking a functional LdeK1 by modulating the immune response towards host protection makes it a potential vaccine candidate against Leishmaniasis.

中文翻译:

eIF2α激酶LdeK1的利什曼原虫donovani显性负突变引发宿主保护性免疫反应。

LdeK1基因(来自利什曼原虫的eIF2α激酶)的显性负突变揭示了其在较早地响应营养饥饿的翻译调控中的作用。然而,该激酶是否会影响其生命周期中自然会遇到营养剥夺的寄生虫的传染性,这值得研究。体外和体内实验都分别降低了腹膜巨噬细胞和脾/肝负荷中的寄生虫负担。对感染突变寄生虫的小鼠的免疫反应的深入了解表明,促炎性细胞因子和一氧化氮水平增强,但TH 2和Treg种群减少。
更新日期:2019-11-01
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