We have studied the intracellular localization of annexins I,II, VI, VII, and XI in cells containing latex beads or Mycobacterium avium at different times after ingestion in order to establish whether a correlation existed between the association of annexins to phagosomes and phagolysosomal fusion, since the intracellular survival of mycobacteria is linked to an impairment of phagosome maturation. We demonstrate an important decrease in the levels of association of annexins I, VI, VII and XI, but not II to phagosomes containing either live or killed mycobacteria compared with phagosomes containing inert latex particles. The reduced association of annexins observed was detected only on M. avium-containing phagosomes and not in other cell membrane nor in cytosolic fractions from infected cells, and was apparent from 8 hours through to 4 days after phagocytosis. These findings add elements to the present knowledge of the phagosomal modifications that accompany the survival of intracellular pathogens, suggesting that annexins I, VI, VII, and XI play a secondary role in phagosomal fusion events while annexin II does not seem to be related to the mechanism of regulation of endolysosomal fusion.

中文翻译：

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含分枝杆菌的吞噬体将较少的膜联蛋白I，VI，VII和XI结合在一起，但吞噬溶酶体融合减少，但没有结合II。

我们研究了吞噬后不同时间膜联蛋白I，II，VI，VII和XI在含有乳胶珠或鸟分枝杆菌的细胞中的细胞内定位，以便确定膜联蛋白与吞噬体的结合与吞噬体融合之间是否存在相关性，因为分枝杆菌的细胞内存活与吞噬体成熟的损害有关。我们证明与含有惰性乳胶颗粒的吞噬体相比，膜联蛋白I，VI，VII和XI（而非II）与含有活或杀死的分枝杆菌的吞噬体的缔合水平显着降低。仅在含鸟分枝杆菌的吞噬体上检测不到膜联蛋白的缔合，而在其他细胞膜或感染细胞的胞浆级分中均未检测到。在吞噬作用后的8小时到4天之间是明显的。这些发现为细胞内病原体存活伴随的吞噬体修饰的现有知识增添了一些知识，这表明膜联蛋白I，VI，VII和XI在吞噬体融合事件中起次要作用，而膜联蛋白II似乎与吞噬体融合事件无关。溶酶体融合的调控机制。