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Asymmetrical development of the monoamine systems in 2,4-dichlorophenoxyacetic acid treated rats.
NeuroToxicology ( IF 3.4 ) Pub Date : 2003-02-05 , DOI: 10.1016/s0161-813x(02)00156-0
Analía Bortolozzi 1 , Ricardo Duffard , Ana María Evangelista de Duffard
Affiliation  

The purpose of this study was to determine whether the regional brain biogenic amine levels in adult rats were altered by pre- and post-natal exposure to 2,4-dichlorophenoxyacetic acid (2,4-D). Pregnant rats were daily orally exposed to 70 mg/kg per day of 2,4-D from gestation day (GD) 16 to post-partum day (PPD) 23. After weaning, the pups were assigned to one of two subgroups: T1 fed with untreated diet up to post-natal day (PND) 90 and T2 (maintained with 2,4-D diet up to PND 90). In addition, we wanted to know the effect of 2,4-D on lateralization in the monoamine systems of the basal ganglia of these adult rats and whether there was any correlation with the behavioral developmental pattern previously reported by us. In this study the content of noradrenaline (NA) was significantly increased in substantia nigra (SN) while it decreased in cerebellum in male and female rats of T2 group. The decreased dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and homovallinic acid (HVA) contents in cerebellum, midbrain, ventral tegmental area (VTA) and prefrontal cortex (PFc) showed an alteration in the mesocorticolimbic system. However, an increase of DA in SN and of DOPAC and HVA in nucleus accumbens (NAc) in both sexes and of DA and DOPAC (only in females) in striatum was detected. The contents of serotonin (5-hydroxytryptamine, 5-HT) were significantly increased in both sexes in PFc, striatum (St), midbrain, SN and cerebellum. Variations of any monoamine levels in NAc and VTA were determined. T1 rats were irreversibly altered: a diminution in DA and/or DOPAC levels in PFc, midbrain, VTA and cerebellum was determined. Indolamines of these rats were increased in both sexes in PFc and St. There was also a large increase in 5-HT levels in midbrain of male rats. Although no changes in the dopaminergic system with respect to their control values in any side of these brain structures were observed, DA and DOPAC levels were found to be decreased in the right side with respect to the left side in striata and accumbens nuclei in T2 female rats supporting the behavioral rotation previously registered by us in these rats. In addition, the increased 5-HT content detected in both the right and left striata observed in this study could be the answer to the behaviors observed and to the early alterations in dopamine in basal ganglia by 2,4-D in neonatal exposed rats, mediated by a serotonergic modulation on the dopaminergic system.

中文翻译:

在2,4-二氯苯氧基乙酸处理的大鼠中单胺系统的不对称发展。

这项研究的目的是确定成年大鼠在出生前和出生后暴露于2,4-二氯苯氧基乙酸(2,4-D)是否会改变区域性脑生物胺水平。从妊娠第16天到产后第23天,每天从妊娠大鼠中每天口服70 mg / kg的2,4-D暴露,在断奶后将幼仔分为两个亚组之一:T1喂养直至出生后一天(PND)90和T2的未经治疗的饮食(维持2,PN饮食直至PND 90的饮食)。此外,我们想知道2,4-D对这些成年大鼠基底神经节单胺系统侧向化的影响,以及与我们先前报道的行为发展模式是否相关。在这项研究中,T2组的雄性和雌性大鼠黑质中去甲肾上腺素(NA)的含量均显着增加,而小脑中则降低。小脑,中脑,腹侧被盖区(VTA)和前额叶皮层(PFc)中的多巴胺(DA),3,4-二羟基苯乙酸(DOPAC)和高缬氨酸(HVA)含量降低,表明中皮质皮质系统改变。但是,检测到两性中SN的DA以及伏隔核(NAc)中的DOPAC和HVA的增加以及纹状体中DA和DOPAC(仅在女性中)的增加。PFc,纹状体(St),中脑,SN和小脑的两性中5-羟色胺(5-羟色胺,5-HT)的含量均显着增加。确定了NAc和VTA中任何单胺含量的变化。T1大鼠不可逆转地改变:测定了PFc,中脑,VTA和小脑中DA和/或DOPAC水平的降低。这些大鼠的吲哚胺在PFc和St的性别均增加。雄性大鼠中脑的5-HT水平也大量增加。尽管在这些脑部结构的任何一侧均未观察到多巴胺能系统相对于其控制值的变化,但在T2雌性中,纹状体和伏隔核的右侧相对于左侧的DA和DOPAC水平降低了支持我们先前在这些大鼠中注册的行为旋转的大鼠。此外,在这项研究中观察到的在左右纹状体中检测到的5-HT含量增加可能是观察到的行为的答案,以及在暴露于新生大鼠的2,4-D对基底神经节中多巴胺的早期改变的答案,
更新日期:2019-11-01
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