We investigated the potential anticancer effects of oleocanthal (OC) on neuroblastoma cells. Cells were divided into four groups: group 1, neuroblastoma cells were treated with OC; group 2, neurons that differentiated from neuroblastoma cells were treated with phosphate-buffered saline(PBS); group 3, bone marrow derived neuronal (BMDN) cells that were differentiated from bone marrow derived mesenchymal stem cells (BMSCs) were treated with OC; group 4, BMDN cells that were differentiated from BMSCs were treated with PBS. Groups 2 and 4 were control groups. The effects of OC on cell viability, oxidative stress, neurite inhibition and apoptosis at IC50 dose were investigated using MTT analysis, i-NOS and e-NOS measurement, neurotoxicity screening test (NST) and TUNEL staining, respectively. MTT analysis demonstrated that cells were significantly less viable in group 1 than in group 3. i-NOS and e-NOS staining intensity was significantly greater in group 1 than in group 3. NST revealed that OC inhibited neurite growth in both neuroblastoma and BMND cells; inhibition was significantly less in group 3 than in group 1. Significantly more TUNEL labeled cells were found in group 1 than in group 3. We found that OC prevented growth and proliferation of neuroblastoma cells in culture by increasing oxidative stress and apoptosis. We also found that the cytotoxicity of OC is negligible in BMDN cells.

中文翻译：

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油橄榄素对培养物中神经母细胞瘤癌细胞增殖的抑制作用。

我们调查了油酸（OC）对神经母细胞瘤细胞的潜在抗癌作用。将细胞分为四组：第1组，用OC处理神经母细胞瘤细胞；用OC处理细胞。第2组，用磷酸盐缓冲液（PBS）处理分化为神经母细胞瘤细胞的神经元。第3组，用OC处理与骨髓间充质干细胞（BMSCs）分化的骨髓源神经元（BMDN）细胞。第4组，用PBS处理自BMSCs分化的BMDN细胞。组2和4为对照组。使用MTT分析，i-NOS和e-NOS测量，神经毒性筛选试验（NST）和TUNEL染色分别研究了IC50剂量下OC对细胞活力，氧化应激，神经突抑制和凋亡的影响。MTT分析表明，第1组的细胞活力明显低于第3组。第1组的i-NOS和e-NOS染色强度明显高于第3组。NST表明OC抑制了神经母细胞瘤和BMND细胞的神经突生长。 ; 第3组的抑制作用明显少于第1组。第1组的TUNEL标记细胞明显多于第3组。我们发现OC通过增加氧化应激和凋亡来阻止培养物中神经母细胞瘤细胞的生长和增殖。我们还发现在BMDN细胞中OC的细胞毒性可忽略不计。第3组的抑制作用明显少于第1组。第1组的TUNEL标记细胞明显多于第3组。我们发现OC通过增加氧化应激和凋亡来阻止培养物中神经母细胞瘤细胞的生长和增殖。我们还发现在BMDN细胞中OC的细胞毒性可忽略不计。第3组的抑制作用明显少于第1组。第1组的TUNEL标记细胞明显多于第3组。我们发现OC通过增加氧化应激和凋亡来阻止培养物中神经母细胞瘤细胞的生长和增殖。我们还发现在BMDN细胞中OC的细胞毒性可忽略不计。