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Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways.
Rice ( IF 5.5 ) Pub Date : 2019-10-21 , DOI: 10.1186/s12284-019-0334-6
Dagang Jiang 1 , Lingyan Zhou 1, 2 , Weiting Chen 1 , Nenghui Ye 3 , Jixing Xia 4 , Chuxiong Zhuang 1
Affiliation  

Background

The NAC (NAM, AFAT, and CUC) transcription factors play critical roles in rice (Oryza sativa) development and stress regulation. Overexpressing a microRNA (miR164b)-resistant OsNAC2 mutant gene, which generates transcripts that cannot be targeted by miR164b, improves rice plant architecture and yield; however, the performance of these mOsNAC2-overexpressing lines, named ZUOErN3 and ZUOErN4, under abiotic stress conditions such as drought have not yet been fully characterized.

Results

In this study, we showed that the germination of ZUOErN3 and ZUOErN4 seeds was delayed in comparison with the wild-type (WT) seeds, although the final germination rates of all lines were over 95%. The quantification of the endogenous ABA levels revealed that the germinating mOsNAC2-overexpressing seeds had elevated ABA levels, which resulted in their slower germination. The mOsNAC2-overexpressing plants were significantly more drought tolerance than the WT plants, with the survival rate increasing from 11.2% in the WT to nearly 70% in ZUOErN3 and ZUOErN4 plants after a drought treatment. Salt (NaCl) tolerance was also increased in the ZUOErN3 and ZUOErN4 plants due to significantly increased ABA levels. A reverse transcription quantitative PCR (RT-qPCR) analysis showed a significant increase in the expression of the ABA biosynthesis genes OsNCED1 and OsNCED3 in the mOsNAC2-overexpressing lines, and the expression levels of the stress-responsive genes OsP5CS1, OsLEA3, and OsRab16 were significantly increased in these plants. Moreover, OsNAC2 directly interacted with the promoters of OsLEA3 and OsNCED3 in yeast one-hybrid assays.

Conclusions

Taken together, our results show that OsNAC2 plays a positive regulatory role in drought and salt tolerance in rice through ABA-mediated pathways.


中文翻译:

针对microRNA的NAC转录因子的过表达通过ABA介导的途径改善了水稻的干旱和耐盐性。

背景

NAC(NAM,AFAT和CUC)转录因子在水稻(Oryza sativa)发育和胁迫调节中起关键作用。过表达耐microRNA(miR164b)的OsNAC2突变基因会产生无法被miR164b靶向的转录本,从而提高了水稻的植株结构和产量;但是,尚未充分表征这些mOsNAC2过表达株ZUOErN3和ZUOErN4在非生物胁迫条件下的性能,例如干旱。

结果

在这项研究中,我们显示与野生型(WT)种子相比,ZUOErN3和ZUOErN4种子的发芽被延迟,尽管所有品系的最终发芽率均超过95%。内源ABA水平的定量显示,发芽的mOsNAC2过表达种子具有升高的ABA水平,这导致其发芽较慢。该mOsNAC2干旱处理后,过表达植物的耐旱性显着高于野生型植物,存活率从野生型植物的11.2%提高到ZUOErN3和ZUOErN4植物的近70%。由于ABA水平显着提高,ZUOErN3和ZUOErN4植物的耐盐(NaCl)能力也有所提高。反转录定量PCR(RT-qPCR的)分析显示在ABA生物合成基因的表达的增加显著OsNCED1OsNCED3mOsNAC2 -overexpressing线和应力应答基因的表达水平OsP5CS1OsLEA3OsRab16这些植物中的植物显着增加。此外,在酵母一杂交试验中,OsNAC2OsLEA3OsNCED3的启动子直接相互作用。

结论

两者合计,我们的结果表明OsNAC2通过ABA介导的途径在水稻的干旱和耐盐性中发挥积极的调节作用。
更新日期:2019-10-21
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