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Fabrication of Thymoquinone-Loaded Albumin Nanoparticles by Microfluidic Particle Synthesis and Their Effect on Planarian Regeneration.
Macromolecular Bioscience ( IF 4.6 ) Pub Date : 2019-10-14 , DOI: 10.1002/mabi.201900182
Aslihan Kazan 1 , Ozlem Yesil-Celiktas 1, 2 , Yu Shrike Zhang 2
Affiliation  

Thymoquinone is the main bioactive component of the plant Nigella sativa, which is commonly known as black seeds and has several therapeutic effects. However, clinical applications of thymoquinone are limited due to its hydrophobic nature. In this study, thymoquinone is encapsulated in albumin nanoparticles by using a microfluidic platform to overcome this limitation. The mean particle sizes of empty and thymoquinone‐loaded nanoparticles are determined as 271.3 and 315.6 nm, respectively, with polydispersity index values both lower than 0.25. In addition to particle size distribution measurements, characterizations of the prepared nanoparticles such as zeta potential measurements, in vitro release studies, as well as scanning electron microscopy, Fourier‐transform infrared, and differential scanning calorimetry analyses are also carried out. To determine the effect of thymoquinone on neural regeneration, planarians are used as the model organism. After application of free and encapsulated thymoquinone, planarians are amputated and the fragments are observed in terms of head and tail regeneration, swimming pattern, and behavior. The results indicate that thymoquinone affects their behavior and primarily enhances head regeneration of planarians. In addition, it is shown that encapsulation of thymoquinone not only enhances the thermal stability of the molecule but also decreases its toxicity.

中文翻译:

微流控粒子合成法制备胸腺嘧啶负载白蛋白纳米粒子及其对平面再生的影响。

胸腺醌是植物Nigella sativa的主要生物活性成分。,通常称为黑种子,具有多种治疗作用。然而,由于其疏水性,胸腺醌的临床应用受到限制。在这项研究中,通过使用微流体平台克服了这一局限性,将胸腺醌包裹在白蛋白纳米颗粒中。确定的空颗粒和载有醌的纳米颗粒的平均粒径分别为271.3和315.6 nm,多分散指数值均低于0.25。除粒度分布测量外,还对制备的纳米颗粒进行了表征,例如ζ电位测量,体外释放研究以及扫描电子显微镜,傅里叶变换红外和差示扫描量热分析。为了确定胸腺醌对神经再生的作用,涡虫被用作模型生物。施用游离的和封装的胸腺醌后,切除平面虫,观察头和尾的再生,游泳方式和行为方面的碎片。结果表明,胸腺醌会影响其行为,并主要增强涡虫的头部再生。另外,显示胸腺醌的包封不仅增强了分子的热稳定性,而且降低了其毒性。
更新日期:2019-10-14
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