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Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan.
Macromolecular Bioscience ( IF 4.6 ) Pub Date : 2019-09-18 , DOI: 10.1002/mabi.201900181
Gurunath Apte 1 , Alexandros Repanas 1 , Christian Willems 1 , Anas Mujtaba 2 , Christian E H Schmelzer 2 , Ashok Raichur 3, 4 , Frank Syrowatka 5 , Thomas Groth 1, 6
Affiliation  

Freestanding multilayer films prepared by layer‐by‐layer technique have attracted interest as promising materials for wound dressings. The goal is to fabricate freestanding films using chitosan (CHI) and alginate (ALG) including subsequent crosslinking to improve the mechanical properties of films while maintaining their biocompatibility. Three crosslinking strategies are investigated, namely use of calcium ions for crosslinking ALG, 1‐ethyl‐3‐(‐3‐dimethylaminopropyl) carbodiimide combined with N‐hydroxysuccinimide for crosslinking ALG with CHI, and Genipin for crosslinking chitosan inside the films. Different characteristics, such as surface morphology, wettability, swelling, roughness, and mechanical properties are investigated showing that films became thinner, exhibited rougher surfaces, had lower water uptake, and increased mechanical strength after crosslinking. Changes of wettability are moderate and dependent on the crosslinking method. In vitro cytotoxicity and cell attachment studies with human dermal fibroblasts show that freestanding CHI‐ALG films represent a poorly adhesive substratum for fibroblasts, while studies using incubation of plastic‐adherent fibroblast beneath floating films show no signs of cytotoxicity in a time frame of 7 days. Results from cell experiments combined with film characteristics after crosslinking, indicate that crosslinked freestanding films made of ALG and CHI may be interesting candidates for wound dressings.

中文翻译:

不同交联策略对由藻酸盐和壳聚糖制成的独立式多层膜的物理性能和生物相容性的影响。

通过多层技术制备的独立式多层膜作为伤口敷料的有前途的材料引起了人们的兴趣。目标是使用壳聚糖(CHI)和藻酸盐(ALG)制作独立的薄膜,包括随后的交联以改善薄膜的机械性能,同时保持其生物相容性。研究了三种交联策略,即使用钙离子交联ALG,1-乙基-3-(-3-甲基二甲基氨基丙基)碳二亚胺与氮的结合‐羟基琥珀酰亚胺可将ALG与CHI交联,而Genipin可使膜内的壳聚糖交联。研究了不同的特性,例如表面形态,润湿性,溶胀性,粗糙度和机械性能,这些结果表明薄膜变得更薄,表面更粗糙,吸水率更低,交联后的机械强度更高。润湿性的变化适中,并取决于交联方法。用人类真皮成纤维细胞进行的体外细胞毒性和细胞附着研究表明,独立的CHI-ALG膜对成纤维细胞的粘附性较差,而使用浮膜下粘附塑料的成纤维细胞进行孵育的研究表明,在7天的时间范围内没有细胞毒性的迹象。细胞实验的结果与交联后的薄膜特性相结合,
更新日期:2019-09-18
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