Isolation and characterization of a Ranavirus associated with disease outbreaks in cultured hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) in Guangxi, China,Journal of Aquatic Animal Health

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Isolation and characterization of a Ranavirus associated with disease outbreaks in cultured hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) in Guangxi, China
Journal of Aquatic Animal Health ( IF 1.2 ) Pub Date : 2019-11-06 , DOI: 10.1002/aah.10090
Hehe Xiao _{1,

2} , Mingzhu Liu _{3,

4} , Siqiao Li ₂ , Deqiang Shi ₂ , Donglin Zhu ₂ , Ke Ke ₂ , Youhou Xu ₄ , Dexin Dong ₂ , Libo Zhu ₂ , Qing Yu ₂ , Pengfei Li ₃

Affiliation

An outbreak of suspected iridovirus disease in cultured hybrid grouper (E. fuscoguttatus♀ × E. lanceolatus♂) occurred in Guangxi Province in July, 2018. In this study, grouper iridovirus Guangxi (SGIV-Gx) was isolated from diseased hybrid grouper collected from Guangxi. Cytopathic effects were observed and identified in grouper spleen cells incubated with diseased tissue homogenates after 24 h, and the effects increased at 48 h post-infection. Transmission electron microscopy results showed that viral particles about 200 nm in diameter and with hexagonal profiles were present in the cell cytoplasm of suspected virus-infected cells. SGIV-Gx (Accession number: MK107821) was identified by polymerase chain reaction (PCR) and amplicon sequencing, which showed that this strain was most closely related to Singapore grouper iridovirus (AY521625.1). The detection of SGIV-Gx infection was further supported by novel aptamer (Q2c)-based detection technology. The effects of temperature and pH on viral infectivity were analyzed by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and cell culture. The results indicated that SGIV-Gx was resistant to exposure to pH 5, 7, and 7.5 for 1 h, but its infectivity was remarkably lower at pH 3 and 10 after 1 h. SGIV-Gx was stable for 1 h at 4 and 25 °C but was inactivated after 1 h at 40, 50, and 60 °C.

更新日期：2019-11-06

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