A bacterial isolate, with an optimum growth temperature of about 50 degrees C, was recovered from the hot spring at Egerszalók in Hungary. Phylogenetic analyses using the 16S rRNA gene sequence of strain H-8T indicated that the new organism represented a new genus and species of alpha-1 subclass of the Proteobacteria. The major fatty acids of strain H-8T are 16:0, 18:1 omega7c; the rare fatty acid 19:0 20H cyclo 11,12 is also present. Ubiquinone 9 is the major respiratory quinone, the polar lipids are phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. The new isolate forms red-colored colonies, flocculates in liquid media, is heterotrophic and strictly aerobic. Thiosulfate is oxidized to sulfate, but an increase in biomass could not be measured because of the flocculating behavior. Bacteriochloropyll a was detected by direct spectrophotometric analysis when the organism was grown at 30 degrees C, but could not be detected after growth at 50 degrees C. pufL and pufM genes were present. Heterotrophic growth of strain H-8T occurs on a few carbohydrates, amino acids and organic acids. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we propose that strain H-8T represents a new genus and a new species most closely related to Roseococcus thiosulfatophilus for which we propose the name Rubritepida flocculans.

中文翻译：

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Rubritepida flocculans gen。十一月，sp。nov。，Proteobacteria的alpha-1子类的新的稍微嗜热的成员。

从匈牙利埃格萨洛克（Egerszalók）的温泉中回收了最佳生长温度约为50摄氏度的细菌分离株。使用菌株H-8T的16S rRNA基因序列进行的系统发育分析表明，新生物代表了变形杆菌属的新属和α-1亚类。H-8T菌株的主要脂肪酸是16：0、18：1 omega7c；稀有脂肪酸19：0 20H环11,12也存在。泛醌9是主要的呼吸道醌，其极性脂质除两个未鉴定的氨基脂质外，还包括磷脂酰胆碱，磷脂酰乙醇胺，二磷脂酰甘油和磷脂酰甘油。新的分离物形成红色菌落，在液体培养基中絮凝，是异养性的且严格需氧的。硫代硫酸盐被氧化成硫酸盐，但是由于絮凝行为，无法测量生物量的增加。当生物体在30摄氏度下生长时，通过直接分光光度法分析可检测到细菌氯吡咯a，但在50摄氏度下生长后无法检测到。存在pufL和pufM基因。H-8T菌株的异养生长发生在一些碳水化合物，氨基酸和有机酸上。根据系统发育分析，生理和生化特征，我们建议菌株H-8T代表一个新的属和一个与硫代硫球菌密切相关的新物种，为此我们将其命名为Rubritepida flocculans。存在pufL和pufM基因。H-8T菌株的异养生长发生在一些碳水化合物，氨基酸和有机酸上。根据系统发育分析，生理和生化特征，我们建议菌株H-8T代表一个新的属和一个与硫代硫球菌最密切相关的新物种，我们将其命名为絮凝棉。存在pufL和pufM基因。H-8T菌株的异养生长发生在一些碳水化合物，氨基酸和有机酸上。根据系统发育分析，生理和生化特征，我们建议菌株H-8T代表一个新的属和一个与硫代硫球菌最密切相关的新物种，我们将其命名为絮凝棉。