The Gram negative bacterium Helicobacter pylori is a human pathogen which infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. A systematic, proteome based approach was chosen to detect candidate antigens of H. pylori for diagnosis, therapy and vaccine development and to investigate potential associations between specific immune responses and manifestations of disease. Sera from patients with active H. pylori infection (n = 24), a control group with unrelated gastric disorders (n = 12) and from patients with gastric cancer (n = 6) were collected and analyzed for the reactivity against proteins of the strain HP 26695 separated by two-dimensional electrophoresis. Overall, 310 antigenic protein species were recognized by H. pylori positive sera representing about 17% of all spots separated. Out of the 32 antigens most frequently recognized by H. pylori positive sera, nine were newly identified and 23 were confirmed from other studies. Three newly identified antigens which belong to the 150 most abundant protein species of H. pylori, were specifically recognized by H. pylori positive sera: the predicted coding region HP0231, serine protease HtrA (HP1019) and Cag3 (HP0522). Other antigens were recognized differently by sera from gastritis and ulcer patients, which may identify them as candidate indicators for clinical manifestations. The data from these immunoproteomic analyses are added to our public database (http://www.mpiib-berlin.mpg.de/2D-PAGE). This platform enables one to compile many protein profiles and to integrate data from other studies, an approach which will greatly assist the search for more immunogenic proteins for diagnostic assays and vaccine design.

中文翻译：

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幽门螺杆菌感染的免疫组学及其与胃疾病的关系。

革兰氏阴性细菌幽门螺杆菌是一种人类病原体，可感染胃粘膜并引起导致胃炎，溃疡和癌症的炎症过程。选择了一种基于蛋白质组的系统方法来检测幽门螺杆菌的候选抗原，以进行诊断，治疗和疫苗开发，并研究特定免疫反应与疾病表现之间的潜在关联。收集活动性幽门螺杆菌感染患者（n = 24），无相关胃病的对照组（n = 12）和胃癌患者（n = 6）的血清，并分析其对菌株蛋白的反应性HP 26695通过二维电泳分离。总体而言，幽门螺杆菌阳性血清可识别310种抗原蛋白，约占所有分离斑点的17％。在幽门螺杆菌阳性血清最常识别的32种抗原中，新鉴定出9种，其他研究证实了23种。幽门螺杆菌阳性血清可特异性识别三种新发现的抗原，它们属于幽门螺杆菌的150种最丰富的蛋白质种类：预测的编码区HP0231，丝氨酸蛋白酶HtrA（HP1019）和Cag3（HP0522）。血清与胃炎和溃疡患者对其他抗原的识别方式不同，这可能将它们识别为临床表现的候选指标。这些免疫蛋白质组学分析的数据将添加到我们的公共数据库（http://www.mpiib-berlin.mpg.de/2D-PAGE）。这个平台可让您编辑多种蛋白质图谱并整合其他研究的数据，