The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this study, we developed a new pea protoplast transfection method to investigate ClYVV propagation at the single-cell level. Using this method, we revealed that ClYVV accumulates to similar levels in both ClYVV-susceptible and cyv1-carrying pea protoplasts. Thus, the cyv1-mediated resistance would not suppress intracellular ClYVV replication.

中文翻译：

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三叶草黄色静脉病毒的细胞内增殖不受豌豆（Pisum sativum）的隐性抗性基因cyv1的影响。

豌豆cyv1基因是尚待鉴定的隐性抗性基因，其抑制三叶草黄脉病毒（ClYVV）的感染。先前的研究证实，在携带cyv1的豌豆植物中，ClYVV的细胞间运动受到抑制。但是，cyv1对病毒复制的影响仍然未知。在这项研究中，我们开发了一种新的豌豆原生质体转染方法，以研究ClYVV在单细胞水平上的繁殖。使用这种方法，我们揭示了ClYVV在易受ClYVV感染和携带cyv1的豌豆原生质体中积累到相似的水平。因此，cyv1介导的耐药性不会抑制细胞内ClYVV复制。