The plasma levels of a panel of cytokines and cytokine-associated molecules (IL-1alpha, IL-2, IL-4, IL-6, IL-10, IL-12, IL-15, macrophage colony-stimulating factor (M-CSF), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), soluble IL-2 receptor (sIL-2R), soluble tumor necrosis factor receptor I or II (sTNFRI or II)) were assessed in 56 plasma samples of 13 pediatric patients undergoing hematopoietic stem cell transplantation (HSCT, bone marrow in 12 and cord blood in one) from unrelated donors. Eight patients developed severe (grade III-IV) acute GVHD (aGVHD). The plasma IL-6, IL-10, M-CSF, sTNFRI and II levels were significantly high in the severe aGVHD group compared to the mild aGVHD group (grade 0-II). The plasma IL-15 level increased transiently in the early period following HSCT and remained high in the severe aGVHD group even after 4 weeks following HSCT. Based on analysis of the correlations between the kinetics of the plasma cytokine levels after HSCT and the clinical manifestations of aGVHD, IL-15 and/or M-CSF were involved in the development of aGVHD, following elevation of the plasma IL-10 and sTNFRI or II levels. These kinetics suggest that IL-10 and sTNFRs worked as suppressor cytokines and seemed to suppress clinical manifestations of aGVHD. Furthermore, it seemed that the plasma ratio of IL-10/sTNFRII from 5 to 12 weeks following HSCT was linked to the poor outcome in the patients with severe aGVHD, suggesting that IL-10 plays an important role in protecting hosts from transplantation-related complications, including GVHD.

中文翻译：

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来自无关供体的造血干细胞移植后血浆细胞因子的动力学：血浆IL-10 / sTNFR水平之比作为严重急性移植物抗宿主病的潜在预后标志物。

一组细胞因子和细胞因子相关分子（IL-1alpha，IL-2，IL-4，IL-6，IL-10，IL-12，IL-15，巨噬细胞集落刺激因子（M- CSF），干扰素-γ（IFN-γ），肿瘤坏死因子-α（TNF-alpha），可溶性IL-2受体（sIL-2R），可溶性肿瘤坏死因子受体I或II（sTNFRI或II））从13位来自无关供体的造血干细胞移植（HSCT，12例骨髓和1例脐血）的儿科患者的56份血浆样本中。八名患者发展为严重（III-IV级）急性GVHD（aGVHD）。重度aGVHD组的血浆IL-6，IL-10，M-CSF，sTNFRI和II水平显着高于轻度aGVHD组（0-II级）。在HSCT后早期，血浆IL-15水平瞬时升高，而在严重aGVHD组中，即使在HSCT后4周后，血浆IL-15水平仍保持较高水平。根据HSCT后血浆细胞因子水平动力学与aGVHD的临床表现之间的相关性分析，在血浆IL-10和sTNFRI升高后，IL-15和/或M-CSF参与了aGVHD的发展或II级。这些动力学表明IL-10和sTNFRs起抑制细胞因子的作用，似乎抑制了aGVHD的临床表现。此外，似乎在HSCT后5至12周的IL-10 / sTNFRII血浆比率与重度aGVHD患者的不良预后有关，表明IL-10在保护宿主免于移植相关疾病中起重要作用。并发症，包括GVHD。