Sodium chloride-tolerant calli were selected from leaf-derived embryogenic calli of Dactylis glomerata L. on agar solidified medium supplemented with 200 mM NaCl, a concentration lethal to non-selected calli. Growth characteristics, water relations and proline accumulation pattern were compared in selected and non-selected lines. The objective was to gain an understanding of the mechanism(s) of tolerance in the NaCl-tolerant line. Growth in the selected line, as expressed in terms of tolerance index (ratio of fresh wt. on NaCl medium:fresh wt. on NaCl free medium x 100), was greater than that of the non-selected line at all levels of NaCl between 50 and 300 mM. There was no significant difference in proline accumulation in the selected and non-selected lines. Maintenance of turgor by osmotic adjustment was observed in the non-selected line despite decreased growth. In contrast, the selected line lost either the need or the ability to adjust osmotically. There was little or no increase in symplastic osmolality in the selected line when exposed to NaCl. Presumably, selection was made for a salt-excluding tissue that has lost the ability to accumulate solutes and adjust turgor with NaCl stress.

中文翻译：

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Dactylis glomerata L. NaCl 选择和非选择愈伤组织系的生长、脯氨酸积累和水分关系。

在补充有 200 mM NaCl 的琼脂固化培养基上，从 Dactylis glomerata L. 的叶衍生胚胎发生愈伤组织中选择耐氯化钠愈伤组织，该浓度对非选择的愈伤组织是致命的。比较了选择和非选择品系的生长特性、水分关系和脯氨酸积累模式。目的是了解耐 NaCl 品系中的耐受机制。以耐受指数（NaCl 培养基上的新鲜重量：无 NaCl 培养基上的新鲜重量的比率 x 100）表示的选定品系中的生长，在所有 NaCl 水平下均大于非选定品系的生长。 50 和 300 mM。选定品系和非选定品系中脯氨酸积累没有显着差异。尽管生长下降，但在非选择品系中观察到通过渗透调节维持膨润。相比之下，所选品系失去了渗透调节的需要或能力。当暴露于 NaCl 时，所选系的共塑性渗透压几乎没有增加或没有增加。据推测，选择的是不含盐的组织，该组织已经失去了积累溶质和用 NaCl 压力调整膨胀的能力。