The probability of complete loss of plasmid material from plasmid bearing cells undergoing active growth has been modelled and incorporated into predictions for the dynamic concentrations of plasmid bearing cells in both batch and continuous flow, stirred tank bioreactors. The new model is based on an extension of the well-used model of Imanaka and Aiba [Ann. New York Acad. Sci. 369 (1981) 1] and is relatively easy to use compared to complex structured models. The new model predicts that both accelerating and decelerating rates of plasmid loss occur in both batch and continuous flow operation, and agrees well with data collected here and published earlier by others.

中文翻译：

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批量和连续发酵罐中的质粒不稳定性建模

质粒材料从经历活跃生长的带有质粒的细胞中完全丢失的可能性已经被建模并被纳入对间歇和连续流动、搅拌罐生物反应器中的质粒携带细胞的动态浓度的预测中。新模型基于 Imanaka 和 Aiba [Ann. 纽约学院。科学。369 (1981) 1] 并且与复杂的结构化模型相比相对易于使用。新模型预测，在批处理和连续流操作中都会出现质粒丢失的加速和减速速率，并且与此处收集的数据以及其他人早些时候发布的数据非常吻合。