Eukaroytic RNA-binding proteins (RBPs) recognize and process RNAs through recognition of their sequence motifs via RNA-binding domains (RBDs). RBPs usually consist of one or more RBDs and can include additional functional domains that modify or cleave RNA. Engineered RBPs have been used to answer basic biology questions, control gene expression, locate viral RNA in vivo, as well as many other tasks. Given the growing number of diseases associated with RNA and RBPs, engineered RBPs also have the potential to serve as therapeutics. This review provides an in depth description of recent advances in engineered RBPs and discusses opportunities and challenges in the field. This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein-RNA Recognition RNA Methods > RNA Nanotechnology RNA in Disease and Development > RNA in Disease.

中文翻译：

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工程化的真核RNA结合蛋白作为分子工具和治疗剂的潜力。

真核生物RNA结合蛋白（RBP）通过RNA结合结构域（RBD）识别其序列基序，从而识别并处理RNA。RBP通常由一个或多个RBD组成，并且可以包含修饰或切割RNA的其他功能域。工程改造的RBP已用于回答基本生物学问题，控制基因表达，在体内定位病毒RNA以及许多其他任务。鉴于与RNA和RBP相关的疾病越来越多，工程改造的RBP也具有作为治疗剂的潜力。这篇综述深入地介绍了工程RBP的最新进展，并讨论了该领域的机遇与挑战。本文归类于：RNA与蛋白质和其他分子的相互作用>蛋白质-RNA识别RNA方法>