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Complete genome sequence of aglaonema bacilliform virus (ABV).
Archives of Virology ( IF 2.7 ) Pub Date : 2019-11-02 , DOI: 10.1007/s00705-019-04445-0
R A Alvarez-Quinto 1 , B E L Lockhart 1 , J M Moreno-Martinez 1 , N E Olszewski 2
Affiliation  

Aglaonema bacilliform virus (ABV), a member of the genus Badnavirus in the family Caulimoviridae, is associated with leaf deformation and chlorosis in Aglaonema modestum. The complete genome sequence of a Minnesota isolate of ABV was determined. The ABV genome is 7,178 bp in length and similar in size and organization to those of the members of the genus Badnavirus, containing three open reading frames (ORFs) with the potential to encode three proteins of 14.92, 13.33 and 207.95 kDa, respectively. The last ORF (ORF3) encodes a putative polyprotein with conserved domains, including zinc finger, aspartic protease, reverse transcriptase (RT) and RNase H domains, in that order. Phylogenetic analysis using the amino acid sequence of the ORF3 polyprotein showed that ABV clusters with several isolates of taro bacilliform CH virus (TaBCHV). Pairwise alignment using the highly conserved RT/RNase H region reveals that ABV has the highest level of identity (71%) at the nucleotide level to a Hawaiian isolate of TaBCHV.

中文翻译:

glaonema bacilliform病毒(ABV)的完整基因组序列。

Aglaonema bacilliform病毒(ABV)是Caulimoviridae科的Badnavirus属成员,与Aglaonema modetum的叶片变形和萎黄有关。确定了明尼苏达州ABV分离株的完整基因组序列。ABV基因组的长度为7,178 bp,大小和组织与Badnavirus属的成员相似,包含三个开放阅读框(ORF),分别编码三种蛋白质,分别为14.92、13.33和207.95 kDa。最后一个ORF(ORF3)按顺序编码具有保守域的推定多蛋白,包括锌指,天冬氨酸蛋白酶,逆转录酶(RT)和RNase H域。使用ORF3多蛋白的氨基酸序列进行的系统进化分析表明,ABV簇生有几种芋头杆菌型CH病毒(TaBCHV)分离株。
更新日期:2020-01-13
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