Sixteen sites in the watershed of the South Fork of the Broad River (SFBR) in Northeastern Georgia, USA, were sampled in two seasons to detect Campylobacter. Sites were classified as mostly influenced by forest, pasture, wastewater pollution control plants (WPC) or mixed use. Sampling was repeated in the late spring and late fall for 2 years for a total of 126 samples. Free‐catch water and sediment grab samples were taken at each site; Moore’s swabs were placed for up to 3 days at most sites. A total of 56 isolates of thermophilic Campylobacter were recovered. Thirteen samplings were positive by two or three methods, and 26 samplings were positive by only one method; once by Moore’s swab only and 25 times by free‐catch water only. Campylobacter was detected at 58% of cattle pasture sites, 30% of forested sites and 81% of WPC sites. Twenty‐one of the isolates carried antimicrobial resistance genes, mostly blaOXA‐61. Free‐catch water samples were more efficient than Moore’s swabs or sediment samples for recovery of Campylobacter, which was more likely to be detected in streams near cattle pastures and human communities than in forested land.

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通过三种采样方法从美国乔治亚州东北部河流中回收嗜热弯曲杆菌及其耐药基因

美国佐治亚州东北部布罗德河南叉 (SFBR) 流域的 16 个地点在两个季节进行了采样，以检测弯曲杆菌。场地被归类为主要受森林、牧场、废水污染控制厂 (WPC) 或混合用途影响。在春末和秋末重复取样，为期两年，总共 126 个样本。在每个地点采集自由捕获的水和沉积物样本；摩尔的拭子在大多数地点放置长达 3 天。总共回收了 56 株嗜热弯曲杆菌。13个样本采用两种或三种方法检测呈阳性，26个样本仅采用一种方法检测呈阳性；一次仅用摩尔拭子检测，25 次仅用自由捕获的水检测。58% 的牛牧场、30% 的森林地点和 81% 的 WPC 地点检测到弯曲杆菌。其中 21 个分离株携带抗菌素耐药基因，其中大部分为 blaOXA-61。自由捕获的水样比摩尔拭子或沉积物样品更能有效地回收弯曲杆菌，而弯曲杆菌在牛牧场和人类社区附近的溪流中比在林地中更有可能被检测到。