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Metabolic responses to arbuscular mycorrhizal fungi are shifted in roots of contrasting soybean genotypes.
Mycorrhiza ( IF 3.9 ) Pub Date : 2019-08-14 , DOI: 10.1007/s00572-019-00909-y
María Soraya Salloum 1 , Marina Insani 2 , Mariela Inés Monteoliva 1 , María Florencia Menduni 3 , Sonia Silvente 4 , Fernando Carrari 5, 6 , Celina Luna 1
Affiliation  

Modern breeding programs have reduced genetic variability and might have caused a reduction in plant colonization by arbuscular mycorrhizal fungi (AM). In our previous studies, mycorrhizal colonization was affected in improved soybean genotypes, mainly arbuscule formation. Despite substantial knowledge of the symbiosis-related changes of the transcriptome and proteome, only sparse clues regarding metabolite alterations are available. Here, we evaluated metabolite changes between improved (I-1) and unimproved (UI-4) soybean genotypes and also compare their metabolic responses after AM root colonization. Soybean genotypes inoculated or not with AM were grown in a chamber under controlled light and temperature conditions. At 20 days after inoculation, we evaluated soluble metabolites of each genotype and treatment measured by GC-MS. In this analysis, when comparing non-AM roots between genotypes, I-1 had a lower amount of 31 and higher amount of only 4 metabolites than the UI-4 genotype. When comparing AM roots, I-1 had a lower amount of 36 and higher amount of 4 metabolites than UI-4 (different to those found altered in non-AM treated plants). Lastly, comparing the AM vs non-AM treatments, I-1 had increased levels of three and reduced levels of 24 metabolites, while UI-4 only had levels of 12 metabolites reduced by the effect of mycorrhizas. We found the major changes in sugars, polyols, amino acids, and carboxylic acids. In a targeted analysis, we found lower levels of isoflavonoids and alpha-tocopherol and higher levels of malondialdehyde in the I-1 genotype that can affect soybean-AM symbiosis. Our studies have the potential to support improving soybean with a greater capacity to be colonized and responsive to AM interaction.

中文翻译:

大豆基因型不同的根部对丛枝菌根真菌的代谢反应发生了变化。

现代育种计划降低了遗传变异性,并可能导致丛枝菌根真菌(AM)导致植物定植减少。在我们以前的研究中,菌根定植受到改良大豆基因型(主要是丛枝形成)的影响。尽管对转录组和蛋白质组与共生相关的变化有充分的了解,但只有很少的关于代谢物改变的线索。在这里,我们评估了改良的(I-1)和未经改良的(UI-4)大豆基因型之间的代谢物变化,还比较了AM根定殖后它们的代谢反应。接种或未接种AM的大豆基因型在控制的光照和温度条件下在室内生长。接种后20天,我们评估了每种基因型的可溶性代谢物,并通过GC-MS进行了处理。在此分析中,当比较基因型之间的非AM根时,I-1的含量低于UI-4基因型,而I-1的含量较低,只有4种代谢物。比较AM根时,与UI-4相比,I-1的36个代谢物含量较低,而4种代谢物的含量较高(与未AM处理的植物中发现的那些代谢物不同)。最后,将AM和非AM治疗进行比较,I-1的3种代谢物水平升高,而24种代谢物的水平降低,而UI-4仅因菌根作用而降低了12种代谢物的水平。我们发现糖,多元醇,氨基酸和羧酸发生了重大变化。在针对性分析中,我们发现I-1基因型中的异黄酮和α-生育酚含量较低,而丙二醛含量较高,这可能会影响大豆与AM的共生。
更新日期:2019-08-14
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