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Repeated fruiting of Japanese golden chanterelle in pot culture with host seedlings.
Mycorrhiza ( IF 3.9 ) Pub Date : 2019-07-24 , DOI: 10.1007/s00572-019-00908-z
Wakana Ogawa 1 , Yumi Takeda 2 , Naoki Endo 1, 3 , Shozo Yamashita 4 , Toshiharu Takayama 4 , Masaki Fukuda 1, 2 , Akiyoshi Yamada 1, 2, 5
Affiliation  

Yellow chanterelles are among the most popular wild edible ectomycorrhizal mushrooms worldwide. The representative European golden chanterelle, Cantharellus cibarius, has only once been reported to fruit under greenhouse conditions, due to the difficulty of establishing pure culture. Recently, we developed a new technique for establishing a pure culture of a Japanese golden chanterelle (Cantharellus anzutake), and conducted in vitro ectomycorrhizal synthesis using established strains and Pinus densiflora. Acclimated pine mycorrhizal seedlings colonized with C. anzutake in a pot system under laboratory conditions produced small but distinct basidiomata with developed basidiospores. C. anzutake mycorrhizae were established on Quercus serrata seedlings by inoculation of mycorrhizal root tips of the fungus synthesized on P. densiflora. A scaled-up C. anzutake–host system in larger pots (4 L soil volume) exhibited repeated fruiting at 20–24 °C under continuous light illumination at 150 μmol m−2 s−1 during a 2-year incubation period. Therefore, a C. anzutake cultivation trial is practical under controlled environmental conditions.

中文翻译:

在带有宿主幼苗的盆栽中反复收获日本金鸡油菌。

黄蘑菇是全球最受欢迎的野生食用菌根菌。具有代表性的欧洲金鸡油菌Cantharellus cibarius,由于难以建立纯净的文化,仅在温室条件下才有报道。最近,我们开发了一种新技术,用于建立日本金鸡菌(Cantharellus anzutake)的纯培养物,并使用已建立的菌株和Pinus densiflora进行体外外生菌根合成。在实验室条件下,盆栽系统中被安祖假单胞菌定居的驯化的松树菌根苗产生了小的但独特的担子,带有发达的担子孢子。an竹通过接种致病菌(P. densiflora)上真菌的菌根根尖,在锯齿栎幼苗上建立菌根。在2年的孵化期中,在较大的花盆(土壤体积4 L)中按比例放大的按蚊寄主寄主系统在20–24°C,150μmolm -2  s -1的连续光照下表现出反复结果。因此,在控制的环境条件下,安竹假蝇的栽培试验是可行的。
更新日期:2019-07-24
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