Climatic constraints on insects in alpine environments have important consequences for the biology of their plant mutualists; in particular, reduced insect diversity and activity in alpine plant populations can result in pollinator‐limited seed set and, potentially, in low genetic diversity. However, highly effective pollination by bumblebees in alpine populations can compensate for low visitation rates. In this study we hypothesized that, because of highly effective pollination by bumblebees, alpine populations of Campanula rotundifolia would not experience more frequent cycles of pollinator limitation than low‐elevation populations and would therefore exhibit comparable levels of genetic variability and inbreeding to those found in foothill populations. Enzyme electrophoresis was used to assess genetic variability at nine putative loci in alpine and foothill populations of C. rotundifolia in Colorado. Genetic variability in C. rotundifolia was found to be comparable to that reported for other long‐lived herbaceous perennials. Measures of genetic variability and fixation indices did not differ between high‐ and low‐elevation populations and were consistent with Hardy‐Weinberg expectations. Nonsignificant FST values indicated no genetic differentiation among all populations.

中文翻译：

---

Campanula rotundifolia（桔梗科）高山和山麓种群的遗传多样性

高山环境中昆虫的气候限制对其植物互惠者的生物学产生重要影响；特别是，高山植物种群中昆虫多样性和活动的减少会导致传粉媒介有限的种子集，并可能导致低遗传多样性。然而，高山种群中大黄蜂的高效授粉可以弥补低访问率。在这项研究中，我们假设，由于大黄蜂的高效授粉，轮叶风铃草的高山种群不会比低海拔种群经历更频繁的传粉限制循环，因此会表现出与山麓种群相当的遗传变异和近亲繁殖水平人口。酶电泳用于评估科罗拉多州圆叶 C. rotundifolia 高山和山麓种群的 9 个假定位点的遗传变异性。发现 C. rotundifolia 的遗传变异性与其他长寿草本多年生植物报道的遗传变异性相当。遗传变异性和固定指数的测量在高海拔和低海拔人群之间没有差异，并且与 Hardy-Weinberg 预期一致。不显着的 FST 值表明所有种群之间没有遗传差异。遗传变异性和固定指数的测量在高海拔和低海拔人群之间没有差异，并且与 Hardy-Weinberg 预期一致。不显着的 FST 值表明所有种群之间没有遗传差异。遗传变异性和固定指数的测量在高海拔和低海拔人群之间没有差异，并且与 Hardy-Weinberg 预期一致。不显着的 FST 值表明所有种群之间没有遗传差异。