This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LB_malt) resulted in higher cell density (OD₆₂₀ = 6.6) than use of MB_malt (OD₆₂₀ = 1.7). Sequential batch cultivation increased the cell density threefold (OD₆₂₀ = 20) in LB_malt and eightfold (OD₆₂₀ = 14) in MB_malt. In both single and sequential batches, the production of carotenoids and EPSs using LB_malt was detected in the exponential phase and stationary phase, respectively, while in MB_malt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (Q_E) of 0.67 (mg/L h) in MB_malt and the polymer contained xylose. In LB, Q_E was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.

中文翻译：

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罗氏海藻DSM 16675的栽培技术开发。

这项工作介绍了使用溶原性肉汤（LB）和海洋肉汤（MB）在生物反应器中生产R. marinus DSM 16675及其胞外多糖（EPS）和类胡萝卜素的分批，补料分批和顺序分批培养技术的评估，在两种情况下分别补充了10 g / L的麦芽糖。使用补充了麦芽糖的LB（LB_麦芽）的分批培养 比使用MB_麦芽（OD ₆₂₀  = 1.7）导致更高的细胞密度（OD ₆₂₀ = 6.6 ）。顺序分批培养将 LB_麦芽的细胞密度提高了三倍（OD ₆₂₀ = 20  ），将MB_麦芽的细胞密度提高了八倍（OD ₆₂₀ = 14）。在单批次和连续批次中，分别在指数期和固定期使用LB_麦芽检测类胡萝卜素和EPS的产量，而在MB_麦芽中，在培养的指数期和固定期均可检测到两种产物的形成。在MB_麦芽中以0.67（mg / L h）的总体积生产率（Q _E）产生了异聚_EPS，并且该聚合物包含木糖。在LB中，Q _E较低（0.1 mg / L h），并且在所产生的EPS的组成中未检测到木糖。总而言之，这项研究表明工艺设计和培养基来源对于生产红景天的重要性。 DSM 16675及其代谢物。