The interest in the potential application value of circulating cell free DNA (ccfDNA) has increased rapidly in recent years, as numerous researchers have demonstrated that the change of its level in the blood is associated with many diseases. Its potential role in cancer management is of particular concern. In comparison with traditional invasive tissue biopsy, quantitative analysis of ccfDNA level for the detection of cancer is advantageous due to the non-invasiveness of blood collection. Moreover, its clinical significance in prognosis prediction and dynamic monitoring of disease progression in cancer patients is equally worthy of attention. At the same time, quantitative detection of ccfDNA is being improved to pursue higher sensitivity due to its low concentration in the blood sample. In this review, we will summarize the progress in quantitative technology of ccfDNA and describe the possible relationship between ccfDNA level and cancer diagnosis and prognosis prediction.

近年来，人们对循环无细胞DNA（ccfDNA）的潜在应用价值的兴趣迅速增长，因为许多研究人员已证明血液中其水平的变化与许多疾病有关。它在癌症管理中的潜在作用尤其令人关注。与传统的侵入性组织活检相比，由于血液采集的非侵入性，定量分析ccfDNA水平以检测癌症是有利的。此外，其在癌症患者的预后预测和疾病进展动态监测中的临床意义同样值得关注。同时，由于ccfDNA在血液样品中的浓度较低，因此定量检测的方法正在改进，以追求更高的灵敏度。在这篇评论中