An upsurge in African horse sickness (AHS) in the Eastern Cape, South Africa, from 2006 led to an epidemiological reassessment of the disease there. Light trapping surveys carried out near horses, donkeys and zebras in 2014-2016 collected 39 species of Culicoides midge (Diptera: Ceratopogonidae) that are potential vectors of AHS. To establish if these midges fed on equids, DNA sequences were obtained from the gut contents of 52 female midges (35 freshly blood-fed, 13 gravid and four parous), representing 11 species collected across 11 sites. Culicoides leucostictus fed on all three equids. Culicoides bolitinos, Culicoides imicola and Culicoides magnus fed on both horses and donkeys. Culicoides onderstepoortensis fed on donkeys, and Culicoides similis and Culicoides pycnostictus fed on zebras. Bloodmeals from cows, pigs, warthogs, impalas and a domestic dog were also identified in various species, but none of the midges tested had fed on birds. These results contribute to knowledge of the vectorial capacity of several species of Culicoides with regard to AHS in the Eastern Cape and point to potential reservoir hosts, of which donkeys, zebras and domestic dogs have previously been found to harbour AHS. Blood-fed midges were also obtained throughout winter, indicating the potential for endemic AHS in the province.

中文翻译：

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在南非东开普省的马，驴和斑马附近采集的库库克mid中的血粉分析。

自2006年以来，南非东开普省非洲马病（AHS）激增，导致对该病的流行病学重新评估。2014-2016年在马，驴和斑马附近进行的光诱捕调查收集了39种Culicoides ge（Diptera：Ceratopogonidae），它们是AHS的潜在媒介。为了确定这些mid是否以马为食，从52只雌性g的肠道内容物中获得了DNA序列（35头新鲜血喂养的，13头妊娠的和4头的雌性），代表了从11个地点采集的11种。Culicoides leucostictus喂所有三个马。以马和驴为食的Culicoides bolitinos，Culicoides imicola和Culicoides magnus。Culicoides onderstepoortensis以驴为食，Culicoides similis和Culicoides pycnostictus以斑马为食。牛，猪，疣猪的血粉，黑斑羚和家养狗也被鉴定为各种物种，但是测试的mid均没有以鸟类为食。这些结果有助于人们了解东开普省几种库里科迪斯人对AHS的矢量能力，并指出潜在的水库宿主，以前已经发现其中的驴，斑马和家犬具有AHS。整个冬季也获得了以血液喂养的mid虫，这表明该省可能存在地方性AHS。