Summary Faecal samples were collected from 224 dogs (47 villages) in Ankara. Toxocara spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for Toxocara spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of T.canis eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification T. canis. The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected Toxocara isolates from dogs, 5 (23.8 %) samples were PCR-positive for T. canis, and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect Toxocara canis DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identification based on PCR in faecal examinations need to be investigated further.

中文翻译：

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PCR检测和鉴定感染犬弓首蛔虫

总结 从安卡拉的 224 只狗（47 个村庄）收集了粪便样本。弓蛔虫 使用离心浮选和沉淀方法在粪便中诊断出虫卵。总共 21 只狗 (9.38%) 对弓首蛔虫呈阳性。蛋。在这项研究中，我们使用了 PCR 技术，结合 DNA 测序，可以检测和识别受感染狗的粪便中的犬 T.canis 卵。为此，选择 ATPase subunit-6 基因 (mtDNA) 作为扩增犬毛丝虫的靶标。引物用于扩增 217 bp 区域。在 21 份从狗身上经阴道镜检测到的弓蛔虫分离株中，5 个 (23.8 %) 样本的犬毛弓虫 PCR 呈阳性，其余 16 份样本呈 PCR 阴性。结果表明 PCR 可以检测受感染犬只粪便中的犬弓首蛔虫 DNA，但与沉降/浮选相比，效率较低。PCR 是诊断这种感染的附加测试。但是，在粪便检查中基于 PCR 识别的困难需要进一步研究。