To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3‐GFP, pOBCol3.6‐GFP, and DMP1‐GFP), similar to the endogenous proteins, are also expressed by bone‐forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP‐Cerulean/DMP1‐Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24‐258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1‐Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP‐Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblasts.

中文翻译：

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DSPP-Cerulean/DMP1-Cherry 报告小鼠的生成和表征。

为了更好地了解成牙本质细胞谱系中祖细胞的进展，我们检查并表征了成牙本质细胞分化过程中一系列 GFP 报告基因的表达。然而，之前报道的 GFP 报告基因（pOBCol2.3-GFP、pOBCol3.6-GFP 和 DMP1-GFP）与内源蛋白类似，也由骨形成细胞表达，这使得很难区分这两种细胞类型在各种体内和体外研究中。为了克服这些困难，我们使用小鼠 BAC 克隆 RP24-258g7 的细菌重组策略生成了 DSPP-Cerulean/DMP1-Cherry 转基因小鼠。我们在体内和体外分析了牙齿和骨骼中两种转基因的时间和空间表达。这种转基因动物使我们能够观察成牙本质细胞与周围组织（包括牙髓、成釉细胞和成牙骨质细胞）之间的相互作用。我们的研究表明，DMP1-Cherry 与Dmp1类似，在功能性和完全分化的成牙本质细胞以及成骨细胞、骨细胞和成牙骨质细胞中表达。DSPP-Cerulean 转基因的表达仅限于功能性和完全分化的成牙本质细胞，并与Dspp的表达相关。这种转基因动物可以帮助鉴定和分离分化后期的成牙本质细胞，并有助于更好地了解牙本质和成牙本质细胞的发育障碍。