At the end of 2017 and 2018 two different unknown suspicious preparations were encountered and were subjected to a plethora of different analyses in order to identify, if present, any bioactive compound. It turned out that these samples contained the assumedly cognitive enhancing research peptides Selank and Semax, which, to our knowledge, have not completed any clinical trials. Moreover, an online search, excluding the dark web, demonstrated that these kinds of nootropic research peptides are freely available either as lyophilized powder for injection purposes or are present in nasal sprays. It stands to reason that controlling laboratories need to anticipate the uprising of these types of potentially dangerous molecules and must therefore be able to correctly identify these compounds. Therefore, these findings served as an incentive to develop a novel combined liquid chromatography tandem mass spectroscopy (LC–MS/MS) methodology, applicable to both hydrophilic or more hydrophobic peptides, which was utilized to analyze a total of 10 putative cognitive enhancing polypeptides, with variable biochemical characteristics, that are currently being sold online. The screening rationale, complying to the recommendation paper of the General European Official Medicines Control Laboratory (OMCL) network on the interpretation of screening results for unknown peptides by mass spectrometry, was also validated in different matrices as required by ISO 17025.

中文翻译：

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缉获的药物制剂中公认的增强认知能力的研究肽的出现：激励控制机构为此类未来的遭遇做准备。

在2017年底和2018年底，遇到了两种不同的未知可疑制剂，并对它们进行了大量不同的分析，以鉴定是否存在任何生物活性化合物（如果存在）。事实证明，这些样品包含假定的认知增强研究多肽Selank和Semax，据我们所知，它们尚未完成任何临床试验。此外，除暗网外的在线搜索表明，这类促智研究肽可作为冻干粉用于注射目的而免费提供，也可存在于鼻喷雾剂中。有理由认为，控制实验室需要预见到这类潜在危险分子的起义，因此必须能够正确识别这些化合物。因此，这些发现激励着人们开发出一种新颖的液相色谱串联质谱联用技术（LC-MS / MS），该方法适用于亲水性或更疏水性的肽段，该方法被用于分析总共10种假定的认知增强多肽，生化特征，目前正在网上出售。符合ISO 17025要求的在不同基质中均已验证了筛选依据，该筛选依据符合通用欧洲官方药物管制实验室（OMCL）网络关于通过质谱法对未知肽的筛选结果进行解释的建议文件。用来分析总共10种具有可变的生化特征的假定的认知增强多肽，这些多肽目前正在网上销售。符合ISO 17025要求的在不同基质中均已验证了筛选依据，该筛选依据符合通用欧洲官方药物管制实验室（OMCL）网络关于通过质谱法对未知肽的筛选结果进行解释的建议文件。用来分析总共10种具有可变的生化特征的假定的认知增强多肽，这些多肽目前正在网上销售。符合ISO 17025要求的在不同基质中均已验证了筛选依据，该筛选依据符合通用欧洲官方药物管制实验室（OMCL）网络关于通过质谱法对未知肽的筛选结果进行解释的建议文件。