Enhanced tolerance to wilt disease (Fusarium oxysporum f.sp. cubense) was achieved in banana variety Rasthali (AAB) by the transformation of embryogenic cells with two antimicrobial genes viz., Ace-AMP1 and pflp using Agrobacterium mediated transformation. The transgene copy numbers in stable transformants were confirmed by Southern analysis. The expression of stacked genes in the transgenic lines was validated by RT-PCR as well as Northern analysis. Bioassay using Foc race 1 in pot culture experiments demonstrated enhanced tolerance after 180 days of planting. Two independent transformants showed 10-20% Vascular Discoloration Index compared to untransformed banana cv. Rasthali (96%). The stacked lines revealed higher activity of Super Oxide Dismutase and Peroxidase compared to untransformed control which depicted higher tolerance to oxidative stress caused by Foc infection.

中文翻译：

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香蕉中堆叠的抗菌基因的部署对尖孢镰刀菌稳定耐受性 通过遗传转化获得立方体。

通过使用农杆菌介导的转化，用两个抗菌基因即Ace-AMP1和pflp转化胚胎发生细胞，在香蕉品种Raththali（AAB）中实现了对枯萎病（枯萎镰孢）的增强耐受性。通过Southern分析确认稳定转化体中的转基因拷贝数。通过RT-PCR和Northern分析验证转基因品系中堆叠基因的表达。在盆栽培养实验中使用Foc race 1进行的生物测定表明，种植180天后耐受性增强。与未转化的香蕉简历相比，两个独立的转化子显示出10-20％的血管变色指数。拉斯塔利（96％）。