ABSTRACT Biological staining of tissue is a crucial procedure in histotechnology. Rudimentary methods for section preparation have often used stains from natural products, although use of synthetic dyes has become the contemporary standard. Artificial dyes increase the operating costs of a laboratory as well as increase the environmental and personnel risks during manufacturing and usage. These considerations have stimulated research to find alternative natural stains from the wide diversity of plant species. The present study investigated the effect of Eucalyptus sp. (Myrtaceae) wood waste extract on histological staining of animal tissues, using a series of pigment concentrations, pH conditions, and temperatures. Eucalyptus wooden slivers were dried, milled, and 1 g, 2 g, and 4 g of the fine powder was subjected to 50% ethanol extraction for 2 days. Staining tests were then performed on formalin fixed paraffin embedded (FFPE) sections. Increasing acetic acid concentrations (1%, 2% and 4%) were added to the extracts and compared to an acid-free extract. Staining was performed at both ambient room temperature (RT) and 60°C. Connective tissue acidophilic components were well-contrasted and a hematoxylin counterstain demonstrated distinct structural differences between matrix and cell nuclei. Therefore, the present findings demonstrate the potential utility of the eucalyptus wood extracts application as a natural stain alternative for routine histology.

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桉木废料提取物在结缔组织组织学染色中的适用性

摘要 组织的生物染色是组织技术中的一个关键过程。尽管使用合成染料已成为当代标准，但切片制备的基本方法通常使用天然产品的染色剂。人造染料增加了实验室的运营成本，并增加了制造和使用过程中的环境和人员风险。这些考虑刺激了从广泛的植物物种多样性中寻找替代天然污渍的研究。本研究调查了桉树的影响。（桃金娘科）木材废料提取物对动物组织的组织学染色，使用一系列色素浓度、pH 条件和温度。桉木木条经干燥、研磨、1g、2g、4g细粉用50%乙醇提取2天。然后对福尔马林固定石蜡包埋 (FFPE) 切片进行染色测试。将增加的乙酸浓度（1%、2% 和 4%）添加到提取物中，并与无酸提取物进行比较。在环境室温 (RT) 和 60°C 下进行染色。结缔组织嗜酸成分对比良好，苏木精复染剂显示基质和细胞核之间存在明显的结构差异。因此，本研究结果证明了桉木提取物应用作为常规组织学的天然染色替代品的潜在效用。2% 和 4%）被添加到提取物中并与无酸提取物进行比较。在环境室温 (RT) 和 60°C 下进行染色。结缔组织嗜酸成分对比良好，苏木精复染剂显示基质和细胞核之间存在明显的结构差异。因此，本研究结果证明了桉木提取物应用作为常规组织学的天然染色替代品的潜在效用。2% 和 4%）被添加到提取物中并与无酸提取物进行比较。在环境室温 (RT) 和 60°C 下进行染色。结缔组织嗜酸成分对比良好，苏木精复染剂显示基质和细胞核之间存在明显的结构差异。因此，本研究结果证明了桉木提取物应用作为常规组织学的天然染色替代品的潜在效用。