Anaerobe ( IF 2.3 ) Pub Date : 2019-10-16 , DOI: 10.1016/j.anaerobe.2019.102110 Sucheta Prabhu Matondkar 1 , Chandrashekar Yavagal 1 , Manohar Kugaji 2 , Kishore G Bhat 2
Scardovia wiggsiae has recently been identified as a potential pathogen associated with dental caries. The aim of the present study was to detect and quantify S. wiggsiae from dental plaque samples of children suffering from severe early childhood caries and children who were caries free by employing a real time DNA polymerase chain reaction (Real-time PCR) method. Dental plaque samples were collected from children suffering from severe early childhood caries (S-ECC) (n = 30) and caries free children (CF) (n = 30) reporting to the out-patient clinics of the department of paediatric and preventive dentistry. Plaque samples from each group were subjected to real-time PCR, post DNA extraction. Both the groups showed the presence of the organism S. wiggsiae, however there was a significant difference in its quantification between groups, with the median number being 1.49 × 108 cells per ml in caries free samples compared to 1.40 × 109 cells per ml in S-ECC samples. S. wiggsiae were isolated from nearly all samples of children, both caries free and those suffering from S-ECC. However, their numbers differ drastically in both groups with the scales tipping towards the S-ECC group, proving their association with the disease process in a significant manner. The present study shows significant association of S. wiggsiae in severe early childhood caries.
中文翻译:
从患有严重的早期龋齿和无龋齿的儿童的牙菌斑样品中定量评价金龟子。
斯嘉多维亚金刚草最近被鉴定为与龋齿有关的潜在病原体。本研究的目的是通过实时DNA聚合酶链反应(Real-time PCR)方法从严重的幼儿龋齿患儿和无龋齿患儿的牙菌斑样品中检测和定量假发链霉菌。从患有严重幼儿龋齿(S-ECC)(n = 30)和无龋齿儿童(CF)(n = 30)的儿童中收集牙菌斑样本,并报告给儿科和预防牙科科的门诊诊所。DNA提取后,将每组的噬斑样品进行实时PCR。两组均显示有金黄色葡萄球菌但是,各组之间的定量差异显着,无龋样品中的中位数为每毫升1.49×10 8个细胞,而S-ECC样品中的中位数为每毫升1.40×10 9个细胞。从几乎所有儿童样本中分离出金黄色葡萄球菌,既不含龋齿又患有S-ECC。然而,在两组中,它们的数量截然不同,其比例接近S-ECC组,从而证明它们与疾病过程的关联性显着。本研究显示严重的幼儿龋病中金黄色葡萄球菌的显着关联。