The immobilization of microorganisms has been reported as an alternative to improve the efficiency of processes such as fermentation, anaerobic digestion, bioadsorption, and many others. Since the kinetics of bioprocesses are governed by the adsorbent/adsorbate interaction, it is important to know the mechanisms of interaction between biological materials and supports. This could help to define optimal operating conditions. In this research, the fungus that produces the cellulases, was selected, and the characterization of the interaction between fungal spores and cobalt ferrite magnetic nanoparticles, was performed. In order to select a fungal strain produces cellulase enzymes, a qualitative Congo Red test was carried out with a culture medium rich in carboxymethylcellulose. From five strains, Aspergillus niger was selected. Chitosan coated cobalt ferrite magnetic nanoparticles (CoMNP-C) were synthesized by single-step co-precipitation. The nano-size of CoMNP-C was demonstrated by XRD. The presence of a high content of amino groups (0.144 mM g-1) was observed, that could have an important role in the interaction between nanoparticles and spores. Adsorption kinetic studies were carried out. The pseudo-equilibrium time was estimated as 90 min. Spores adsorption isotherm was obtained with 3.45 mg of synthesized material at 30 °C. It was found that the adsorption of spores may be described by both models (Langmuir and Freundlich), suggesting a homogeneous surface of the nanoparticles and a multilayer adsorption phenomenon. These results can have transcendence in multiple applications based on the studied process.

中文翻译：

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铁氧体钴纳米颗粒与黑曲霉孢子之间的相互作用。

据报道，固定化微生物是提高发酵，厌氧消化，生物吸附等过程效率的替代方法。由于生物过程的动力学受吸附剂/吸附物相互作用的控制，因此重要的是要了解生物材料与载体之间相互作用的机理。这可能有助于定义最佳操作条件。在这项研究中，选择了产生纤维素酶的真菌，并对真菌孢子和钴铁氧体磁性纳米粒子之间的相互作用进行了表征。为了选择产生纤维素酶的真菌菌株，用富含羧甲基纤维素的培养基进行了定性刚果红试验。从五个菌株中，选择黑曲霉。通过一步共沉淀法合成了壳聚糖包覆的钴铁氧体磁性纳米颗粒（CoMNP-C）。XRD证实了CoMNP-C的纳米尺寸。观察到存在高含量的氨基（0.144 mM g-1），这可能在纳米粒子和孢子之间的相互作用中起重要作用。进行吸附动力学研究。拟平衡时间估计为90分钟。用3.45 mg的合成材料在30°C下获得孢子吸附等温线。已经发现，两种模型（Langmuir和Freundlich）都可以描述孢子的吸附，这表明纳米颗粒的表面均匀并且具有多层吸附现象。这些结果可以基于所研究的过程在多个应用中具有超越性。