Genetic improvement of commercially accepted banana cultivars is strongly reliant on the ability to introduce genes that encode important agro-traits such as disease resistance. In most cases this can only be achieved using a transgenic approach. Public and regulatory acceptance of these events would greatly increase with "clean" single copy integration events free of the selectable marker gene and extraneous vector backbone. This would also allow for the successive addition of new genes and traits as they become available. In this study, we used the pMarker Free 1 (pMF1) vector containing the green fluorescent protein (gfp) reporter gene to assess the effectiveness of steroid-inducible recombination and positive/negative dual selection to regenerate transgenic Cavendish banana plants that were potentially free of the selectable marker gene. By examining the interaction of two different Agrobacterium strains with two different cultivars of Cavendish banana, namely Williams and Grand Naine, we describe a transformation and regeneration strategy that successfully produced marker-free, single transgene copy, gfp-expressing events. The system will provide a useful means of serially improving banana into the future.

中文翻译：

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使用类固醇诱导型重组酶平台生产无选择标记基因的卡文迪许香蕉（Musa spp。）。

商业上可接受的香蕉品种的遗传改良在很大程度上依赖于引入编码重要农业特性（如抗病性）的基因的能力。在大多数情况下，这只能使用转基因方法来实现。这些事件的公众和监管接受程度将随着没有选择标记基因和无关载体骨架的“纯净”单拷贝整合事件而大大增加。这也将允许在新基因和性状可用时连续添加它们。在这个研究中，我们使用了包含绿色荧光蛋白（gfp）报告基因的pMarker Free 1（pMF1）载体，评估了类固醇诱导的重组和正/负双重选择来再生可能不含选择标记基因的转基因卡文迪许香蕉植物的有效性。 。通过检查两个不同的农杆菌菌株与卡文迪许香蕉的两个不同品种Williams和Grand Naine的相互作用，我们描述了成功产生无标记，单一转基因拷贝，gfp表达事件的转化和再生策略。该系统将提供一种有用的手段，以在将来逐步改进香蕉。我们描述了成功产生无标记，单一转基因拷贝，gfp表达事件的转化和再生策略。该系统将提供一种有用的手段，以在将来逐步改进香蕉。我们描述了成功产生无标记，单一转基因拷贝，gfp表达事件的转化和再生策略。该系统将提供一种有用的手段，以在将来逐步改进香蕉。