SummaryKaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

中文翻译：

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在体外成熟培养基中补充山奈酚可调节氧化应激并促进随后的体外胚胎发育

摘要山奈酚 (KAE) 是最常见的膳食黄酮醇之一，具有抗癌、抗炎和抗氧化作用等生物活性。虽然之前的研究已经报道了 KAE 对多种细胞的生物学活性，但尚不清楚 KAE 在卵母细胞和胚胎中是否起类似的作用体外文化系统。本研究调查了 KAE 添加到体外孤雌生殖激活后猪卵母细胞胚胎抗氧化能力的成熟。以相关基因的表达、活性氧、谷胱甘肽和线粒体膜电位为标准，研究了山奈酚对猪卵母细胞卵母细胞质量的影响。用 0.1 µm KAE 处理的卵母细胞的囊胚形成率明显高于对照卵母细胞。凋亡相关基因的mRNA水平Caspase-3经 KAE 处理的卵母细胞的囊胚显着低于对照组和胚胎发育相关基因的 mRNA 表达COX2和SOX2与对照组相比，KAE治疗组显着增加。此外，KAE处理后细胞内活性氧水平显着降低，谷胱甘肽水平显着升高。与对照组相比，KAE治疗组线粒体膜电位（ΔΨm）升高，Caspase-3活性显着降低。总之，这些结果表明，KAE 通过抑制猪卵母细胞的氧化应激，有利于改善胚胎发育。