Chlamydia trachomatis is considered as a public health problem due to its high prevalence and increased rates of gynecological disorders. The major outer membrane protein (MOMP) of this bacterium is the most abundant protein in its membrane and has been evaluated not only as a vaccine development candidate but also is used in many diagnostic tests. The MOMP weighs 69 kDa and contains four variable segments (VS 1–4) separated by constant regions. Several research groups have developed recombinant single-variable segments of MOMP expressed in Escherichia coli cytoplasm. But, all variable segments have been used minimally for the diagnosis of a chlamydial infection. In this experiment, the authors obtained the recombinant MOMP of C. trachomatis (rMOMP) in E. coli rMOMP and extracted, purified, and partially characterized it. This was later used to identify anti-Chlamydia trachomatis antibodies in sera of infertile patients by immunodetection assays, enzyme-linked immunosorbent assay (ELISA), and indirect immunofluorescence tests. The ELISA test showed high sensitivity and low specificity of 100 and 58.3%, respectively. The above results obtained were linked to the cross-reactivity of antibodies against C. pneumoniae or C. psittaci. Hence, an evaluation was performed to obtain an optimized test for the diagnosis of C. trachomatis infection.

中文翻译：

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获得基于沙眼衣原体重组蛋白的ELISA测试。

由于沙眼衣原体的高流行和妇科疾病的发病率上升，它被认为是一个公共卫生问题。该细菌的主要外膜蛋白（MOMP）是其膜中最丰富的蛋白，不仅已被评估为疫苗开发的候选药物，而且已用于许多诊断测试中。MOMP的重量为69 kDa，包含四个由恒定区分开的可变片段（VS 1-4）。几个研究小组已经开发出在大肠杆菌细胞质中表达的MOMP重组单变量区段。但是，所有可变节段已很少用于诊断衣原体感染。在该实验中，作者获得了C的重组MOMP 。E中的沙眼（rMOMP）。大肠杆菌rMOMP，并对其进行提取，纯化和部分表征。随后，通过免疫检测测定，酶联免疫吸附测定（ELISA）和间接免疫荧光测定，将其用于鉴定不育患者血清中的抗沙眼衣原体抗体。ELISA测试显示高灵敏度和低特异性，分别为100和58.3％。以上获得的结果与抗C抗体的交叉反应有关。肺炎或Ç。psittaci。因此，进行了评估以获得用于诊断C的优化测试。沙眼 感染。