Human bocavirus 1 (HBoV1) is a parvovirus associated with pneumonia in infants. It has been detected in different tissues, including colorectal tumors. In this study, we investigated whether Caco-2 cell line, derived from human colon cancer, can be utilized as a model for HBoV1 replication. We demonstrate HBoV1 replication in Caco-2 cultures supplemented with DEAE-dextran after inoculation with respiratory material from infected patients presenting with acute respiratory infection. A viral cycle of rapid development is displayed. However, in spite of HBoV1 DNA 4-fold increment in the supernatants and monolayers by day 1, evidencing that the system allows the virus genome replication after the entry occurred, infectious progeny particles were not produced. These results are consistent with an infection that is limited to a single growth cycle, which can be associated to mutations in the NS1 and VP1/VP2 regions of HBoV1 genome. Further research will contribute to fully elucidate these observations.

中文翻译：

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CACO-2细胞系培养物中人博卡病毒1感染。

人博卡病毒1（HBoV1）是与婴儿肺炎相关的细小病毒。它已在包括结直肠肿瘤在内的不同组织中被检测到。在这项研究中，我们调查了源于人类结肠癌的Caco-2细胞系是否可以用作HBoV1复制的模型。我们证明了接种来自急性呼吸道感染的感染患者的呼吸道材料后，在补充有DEAE-葡聚糖的Caco-2培养物中HBoV1复制。显示了快速发展的病毒周期。然而，尽管到第1天HBoV1 DNA在上清液和单层中增加了4倍，证明该系统允许病毒基因组在进入后复制，但并未产生感染性后代颗粒。这些结果与仅限于单个生长周期的感染是一致的，可能与HBoV1基因组的NS1和VP1 / VP2区的突变有关。进一步的研究将有助于充分阐明这些观察结果。