BACKGROUND Genome-wide association studies have implicated dozens of genes with migraine susceptibility, but it remains unclear in which nervous system cell types these genes are expressed. METHODS Using single-cell RNA sequencing data from the central and peripheral nervous system, including the trigeminal ganglion, the expression of putative migraine-associated genes was compared across neuronal, glial and neurovascular cell types within these tissues. RESULTS Fifty-four putative migraine-associated genes were expressed in the central nervous system, peripheral nervous system or neurovascular cell types analyzed. Six genes (11.1%) were selectively enriched in central nervous system cell types, three (5.5%) in neurovascular cell types, and two (3.7%) in peripheral nervous system cell types. The remaining genes were expressed in multiple cell types. CONCLUSIONS Single-cell RNA sequencing of the brain and peripheral nervous system localizes each migraine-associated gene to its respective nervous system tissue and the cell types in which it is expressed. While the majority of migraine-associated genes are broadly expressed, we identified several cell-type-specific migraine-associated genes in the central nervous system, peripheral nervous system, and neurovasculature. Trial registration: not applicable.

中文翻译：

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中枢和外周神经系统细胞类型中的偏头痛相关基因表达。

背景 全基因组关联研究表明数十个基因与偏头痛易感性有关，但尚不清楚这些基因在哪些神经系统细胞类型中表达。方法使用来自中枢和外周神经系统（包括三叉神经节）的单细胞 RNA 测序数据，比较这些组织内神经元、神经胶质和神经血管细胞类型的推定偏头痛相关基因的表达。结果 54 个推定的偏头痛相关基因在中枢神经系统、外周神经系统或神经血管细胞类型中表达。六个基因 (11.1%) 在中枢神经系统细胞类型中被选择性富集，三个 (5.5%) 在神经血管细胞类型中，两个 (3.7%) 在外周神经系统细胞类型中。其余基因在多种细胞类型中表达。结论 大脑和周围神经系统的单细胞 RNA 测序将每个偏头痛相关基因定位到其各自的神经系统组织和表达它的细胞类型。虽然大多数偏头痛相关基因广泛表达，但我们在中枢神经系统、外周神经系统和神经血管系统中发现了几种细胞类型特异性的偏头痛相关基因。试用注册：不适用。我们在中枢神经系统、外周神经系统和神经血管系统中鉴定了几种细胞类型特异性偏头痛相关基因。试用注册：不适用。我们在中枢神经系统、外周神经系统和神经血管系统中鉴定了几种细胞类型特异性偏头痛相关基因。试用注册：不适用。