Advancements in technology and digitization have ushered in novel ways of enhancing tissue-based research via digital microscopy and image analysis. Whole slide imaging scanners enable digitization of histology slides to be stored in virtual slide repositories and to be viewed via computers instead of microscopes. Easier and faster sharing of histologic images for teaching and consultation, improved storage and preservation of quality of stained slides, and annotation of features of interest in the digital slides are just a few of the advantages of this technology. Combined with the development of software for digital image analysis, digital slides further pave the way for the development of tools that extract quantitative data from tissue-based studies. This review introduces digital microscopy and pathology, and addresses technical and scientific considerations in slide scanning, quantitative image analysis, and slide repositories. It also highlights the current state of the technology and factors that need to be taken into account to insure optimal utility, including preanalytical considerations and the importance of involving a pathologist in all major steps along the digital microscopy and pathology workflow.

中文翻译：

---

数字显微镜，图像分析和虚拟载片库。

技术和数字化的进步带来了通过数字显微镜和图像分析增强基于组织的研究的新颖方法。整个载玻片成像扫描仪可以将组织学载玻片的数字化存储在虚拟载玻片存储库中，并可以通过计算机而不是显微镜进行查看。可以更轻松，更快速地共享组织学图像以进行教学和咨询，改进了染色幻灯片的质量的存储和保存，以及在数字幻灯片中标注感兴趣的特征，只是这项技术的一些优势。与数字图像分析软件的开发相结合，数字幻灯片进一步为开发从基于组织的研究中提取定量数据的工具铺平了道路。这篇评论介绍了数字显微镜和病理学，并解决了幻灯片扫描，定量图像分析和幻灯片存储库中的技术和科学考虑。它还强调了技术的现状和确保最佳效用所需要考虑的因素，包括分析前的考虑以及让病理学家参与数字显微镜和病理学工作流程所有主要步骤的重要性。