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Measurement of corticosterone in mice: a protocol for a mapping review.
Laboratory Animals ( IF 2.4 ) Pub Date : 2019-10-26 , DOI: 10.1177/0023677219868499
Cathalijn H C Leenaars 1, 2 , Stevie van der Mierden 1 , Mattea Durst 3 , Vivian C Goerlich-Jansson 2 , Florenza Lüder Ripoli 1 , Lydia M Keubler 1 , Steven R Talbot 1 , Erin Boyle 1 , Anne Habedank 4 , Paulin Jirkof 3 , Lars Lewejohann 4, 5 , Peter Gass 6 , René Tolba 7 , André Bleich 1
Affiliation  

Severity assessment for experiments conducted with laboratory animals is still based mainly on subjective evaluations; evidence-based methods are scarce. Objective measures, amongst which determination of the concentrations of stress hormones, can be used to aid severity assessment. Short-term increases in glucocorticoid concentrations generally reflect healthy responses to stressors, but prolonged increases may indicate impaired welfare. As mice are the most commonly used laboratory animal species, we performed a systematic mapping review of corticosterone measurements in Mus musculus, to provide a full overview of specimen types (e.g. blood, urine, hair, saliva, and milk) and analysis techniques. In this publication, we share our protocol and search strategy, and our rationale for performing this systematic analysis to advance severity assessment. So far, we have screened 13,520 references, and included 5337 on primary studies with measurements of endogenous corticosterone in M. musculus. Data extraction is currently in progress. When finished, this mapping review will be a valuable resource for scientists interested in corticosterone measurements to aid severity assessment. We plan to present the data in a publication and a searchable database, which will allow for even easier retrieval of the relevant literature. These resources will aid implementation of objective measures into severity assessment.

中文翻译:

小鼠中皮质酮的测定:作图综述的方案。

对实验动物进行实验的严重性评估仍主要基于主观评估。基于证据的方法很少。客观措施可用于确定压力激素的浓度,以帮助进行严重程度评估。糖皮质激素浓度的短期升高通常反映出对应激源的健康反应,但长期升高可能表明福利受损。由于小鼠是最常用的实验室动物物种,因此我们对小家鼠中的皮质酮测量进行了系统的制图综述,以全面概述标本类型(例如血液,尿液,头发,唾液和牛奶)和分析技术。在本出版物中,我们分享了我们的协议和搜索策略,以及进行此系统分析以进行严重性评估的基本原理。到目前为止,我们已经筛选了13,520篇参考文献,其中包括5337篇关于测量小家鼠内生皮质酮的初步研究。数据提取目前正在进行中。完成后,此映射审查将成为对皮质酮测量感兴趣的科学家的宝贵资源,以帮助进行严重程度评估。我们计划将数据呈现在出版物和可搜索的数据库中,这将使相关文献的检索更加轻松。这些资源将有助于将客观措施实施到严重程度评估中。对于对皮质酮测量感兴趣的科学家,此映射审查将是宝贵的资源,以帮助进行严重程度评估。我们计划将数据呈现在出版物和可搜索的数据库中,这将使相关文献的检索更加轻松。这些资源将有助于将客观措施实施到严重程度评估中。对于对皮质酮测量感兴趣的科学家,此映射审查将是宝贵的资源,以帮助进行严重程度评估。我们计划将数据呈现在出版物和可搜索的数据库中,这将使相关文献的检索更加轻松。这些资源将有助于将客观措施实施到严重程度评估中。
更新日期:2019-11-01
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